Čes. slov. farm. 2020, 69(1):48-51 | DOI: 10.36290/csf.2020.006

The amino acid and carbohydrate composition of the herb and roots of Smallanthus sonchifolius

Olga V. Demeshko^1,*, Elena V. Krivoruchko¹, Victoria I. Volochai¹, Victoria A. Samoilova¹, Vladimir V. Kovalev²

¹ National University of Pharmacy, Department of Pharmacy, Kharkiv, Ukraine

² National University of Pharmacy, Department of Technology of Drug, Kharkiv, Ukraine

The free and protein-bound amino acid composition of the herb and roots of Smallanthus sonchifolius was analyzed by HPLC method. Fourteen free and fifteen protein-bound amino acids were determined in yacon herb, and three free and fourteen protein-bound amino acids in the roots. Among the free amino acids, proline (0.44 µg/mg) and aspartic acid (0.12 µg/mg) were dominant in the herb and proline (0.28 µg/mg) in the roots. Among the protein-bound amino acids, aspartic acid (18.58 µg/mg), glutamic acid (16.33 µg/mg) and proline (14.52 µg/mg) prevailed in the herb, and proline (3.14 μg/mg) in the roots. Fructose, sucrose and arabinose were identified in free form in the herb of S. sonchifolius applying gas chromatography-mass spectrometry (GC-MS). The polysaccharide complex was obtained from yacon herb, its yield was 5.13 ± 0.09%. Fructose (3.11 µg/mg) was the only monosaccharide identified in the hydrolysate of the obtained complex.

Keywords: Smallanthus sonchifolius (yacon); Amino acids; Carbohydrates; GC-MS HPLC methods

Received: October 24, 2019; Accepted: January 21, 2020; Published: January 1, 2020  Show citation