Čes. slov. farm. 2011, 60(5):237-240

The effect of three different elicitors on sanguinarine production in suspension cultures of a low-morphine variety of the opium poppy (Papaver somniferum L.)

Andrea Balažová^*, Víťazoslava Blanáriková, František Bilka, Andrea Bilková, Hana Kiňová Sepová

Univerzita Komenského v Bratislave, Farmaceutická fakulta, Katedra bunkovej a olekulárnej biológie liečiv, Slovenská republika

This paper is focused on the evaluation of the effect of three different elicitors on sanguinarine production in suspension cultures derived from a low-morphine variety of the opium poppy. The elicitors CdCl₂, methyl jasmonate and a homogenate from the phytopathogenic fungus Botrytis cinerea have been administered into the growth media of 11-day-old suspension cultures. Their effect on the production of sanguinarine was monitored after 24, 48 and 72 h of exposure. The highest sanguinarine production was observed after 48 h of exposure to the biotic elicitor Botrytis cinerea, which increased the sanguinarine production 5.3-times. In the presence of CdCl₂ at a concentration of 1 mmol.l^-1 the content of sanguinarine increased linearly in suspension cultures and after 72 h of elicitation it was 2.3-times higher than in the non-elicited cultures. Opium poppy suspension cultures responded to methyl jasmonate elicitation by a moderate increase in sanguinane production after 24 and 48 h. A significant increase in the production of this secondary metabolite in cell suspension cultures was observed after 72 h of exposure and it was 3-times higher than the production of sanguinarine in non-elicited cultures.

Keywords: sanguinarine; methyl jasmonate; Botrytis cinerea; CdCl₂; elicitation; opium poppy

Received: June 7, 2011; Accepted: July 7, 2011; Published: May 1, 2011  Show citation