Čes. slov. farm. 2004, 53(2):93-95
In vitro reaktivace acetylcholinesterázy inhibované O-isopropylmethylfluorofosfonátem užitím biskvartérního oximu HS-6
- Katedra toxikologie Vojenské lékařské akademie Jana Evangelisty Purkyně, Hradec Králové
Byla vyhodnocena schopnost oximu HS-6 [1-(4-hydroxyiminomethylpyridinium)-3-(4-karbamoylpyridinium)-2-oxa-propan dichlorid] in vitro reaktivovat enzym acetylcholinesterázu inhibovanýnervově paralytickou látkou - sarinem [O-isopropylmethylfluorofosfonát]. Jako referenční látkybyly vybrány reaktivátory 2-PAM [2-hydroxyiminomethyl-1-methylpyridinium chlorid], obidoxim[1,3-bis(4-hydroxyiminomethylpyridinium)-2-oxa-propan dichlorid] a H-oxim HI-6 [1-(2-hydroxyiminomethylpyridinium)-3-(4-karbomoylpyridinium)-2-oxa-propan dichlorid]. Oxim HS-6 se ukázaljako účinný reaktivátor AChE inhibované sarinem. Nedosahuje sice účinnosti oximu jako H-oximHI-6, nicméně je lepší než 2-PAM či obidoxim.
Klíčová slova: acetylcholinesteráza; sarin; HS-6; HI-6; obidoxim; 2-PAM; in vitro
The in vitro Reactivation of O-Isopropylmethylfluorophosphonate - Inhibited Acetylcholinesteraseby Bisquaternary Oxime HS-6
The ability of the oxime HS-6 [1-(4-hydroxyiminomethylpyridinium)-3-(4-carbamoylpyridinium)-2--oxa-propane dichloride] to reactive in vitro the enzyme acetylcholinesterase inhibited by the nerveagent sarin [O-isopropylmethylfluorophosphonate] was evaluated. The reactivators 2-PAM [2-hydroxyiminomethyl-1-methylpyridinium chloride], toxogonin [1,3-bis(4-hydroxyiminomethylpyridinium)-2-oxa-propane dichloride] and H-oxime HI-6 [1-(2-hydroxyiminomethylpyridinium)--3-(4-carbamoylpyridinium)-2-oxa-propane dichloride] were chosen for comparison. The oxime HS-6was an effective reactivator of sarin-inhibited AChE. It is not as effective as the H-oxime HI-6, butit is better than 2-PAM and obidoxime.
Keywords: acetylcholinesterase; sarin; HS-6; HI-6; obidoxime; 2-PAM; in vitro
Zveřejněno: 1. únor 2004 Zobrazit citaci