Čes. slov. farm. 2022, 71(2):65-75 | DOI: 10.5817/CSF2022-2-65

Capillary zone electrophoresis in combination with UV detection for simultaneous determination of tramadol and paracetamol in pharmaceutical and biological samples

Andrea Horniaková¹, Ondrej Štefánik¹, Ivana Čižmárová¹, Michaela Matušková¹, Peter Mikuš^1,2, Juraj Piešťanský^1,2,*

¹ Katedra farmaceutickej analýzy a nukleárnej farmácie, Farmaceutická fakulta Univerzity Komenského, Bratislava, SR

² Toxikologické a antidopingové centrum FaF UK, Bratislava, SR

The aim of the present study is the development and validation of a simple method based on capillary zone electrophoresis coupled with UV detection for simultaneous determination of tramadol and paracetamol in pharmaceutical and biological samples. The background electrolyte was composed of 50 mM ammonium carbonate, which is a type of a non-conventional electrolyte system. The developed method was characterized by suitable validation parameters, such as linearity (coefficient of determination r2 ≥ 0,995), selectivity or the limit of detection at the level of 0.25 - 0.5 μg/ml. Acceptable values of accuracy and precision were obtained, which were in good agreement with the recommended validation guidelines for analysis of pharmaceutical and biological samples. Detection was performed at a wavelength of 200 nm. The developed method was successfully applied to determine tramadol and paracetamol in various dosage forms and in urine biological samples. Achieved results indicate a potential of the method to be integrated in the common quality control processes of drugs and/or in bioanalysis.

Keywords: quality control; capillary zone electrophoresis; UV detection; tramadol; paracetamol; bioanalysis

Grants and funding:

Práca vznikla s podporou grantov APVV-15-0585, VEGA 1/0463/18 a KEGA 027UK-4/2020.

Received: January 9, 2022; Accepted: February 24, 2022; Published: February 1, 2022  Show citation