Czech and Slovak Pharmacy, 2002 (vol. 51), issue 2
Articles
Inhibitors of 5-Lipoxygenase
M. Gápovský, M. Remko
Čes. slov. farm. 2002, 51(2):55-62
Leukotrienes are powerfizl mediators of inflammation processes. Possibilities of their biosynthesis inhibition have been intensively studied during recent two decades aiming to develop new methods of therapy of various inflammatory diseases, particularly asthma. Biosynthesis of all leukotrienes is dependent on the production of the key intermediate LTA4, Transformation of arachidonic acid to LTA4 is catalyzed by the enzyme 5-lipoxygenase and faciliated by FLAP. The present paper discusses the results of recent development of new drugs in this area.
The ACE Inhibitor Captopril in the Light of Recent Clinical Studies
F. Šimko, M. Šimková, L. Kovács
Čes. slov. farm. 2002, 51(2):63-67
Captopril, the classic inhibitor of the angiotensin converting enzyme, was employed in several large clinical studies in retem years. The effect of captopril was compared either with placebo, or captopril was selected as the reference ACE inhibitor for comparison with another therapy. In the classic study SAVE, captopril administered to patients after myocardial infarction with a dysfunction of the left chamber reduced mortality by 19 %. Though in the study ELITE the ATl blocker losartan was more effective to reduce mortality in patients with chronic heart failure than captopril, the larger and mortality-oriented study ELITE II did not demonstrate...
Dried Milk Based Infant Formula and Its Possible Contamination with Pesticide Rssidues
K. Pecníková, J. Tekec, M. Fulmeková
Čes. slov. farm. 2002, 51(2):68-72
Residues of agricultural pesticides have the potential to enter the organism of farm animals and, subsequently, they can be transferred to milk. Infant formulas are manufactured from dried hydrolyzed cos milk. Therefore, attention should be paid to possible contamination of the raw material for the manufacture of infant formulas with pesticide residues, especially those of persistent triazine herbicides. Dried milk based formulas are often the only food of young children in their first months of life. Thus, the control of infant milk formulas for pesticide residues is an important issue.
Effects of Consupren, Carvedilol and BL-443 on Lactate Dehydrogenase Pattern in Tissues of Rats with Cyclosporine Nephropathy
J. Šalplachta, V. Strnadová, J. Csöllei
Čes. slov. farm. 2002, 51(2):73-77
L-Lactate dehydrogenase pattern in tissues was determined by polyacrylamide gel electrophoresis adjusted to be more sensitive to LDl and LD2. Three groups of rats (Wistar) were treated for 18 days with single oral daily doses of 45 mg consuprene/kg body weight to induce cyclosporine nephropathy. Two treated groups were further medicated either with carvedilol or BL-443 in single daily doses of 10 mg/kg b.w., unmedicated rats were given single i.p. daily doses of 1 ml saline. No significant difference in the LD(1-4) isoenzyme pattern in the liver between intact rats, rats with cyclosporine nephropathy, and rats with cyclosporine nephropathy medicated...
Pharmacoeconomics in the Therapy of Peptic Ulcers
T. Tesař, V. Foltán, M. Huorka
Čes. slov. farm. 2002, 51(2):78-83
The great prevalence and chronic nature of peptic ulcers traditionally represent a great economic load for the system of health care. The exponentially growing therapeutic and diagnostic possibilities of the treatment of peptic diseases are under a strong economic pressure from health-insurance companies. The use of an economic model for the management of peptic diseases aims to maximize health benefit from limited sources of the health services. It can be said already now tkat the detection of the Helicobacter pylori infection ranks among the most important discoveries of the 20th century. Elimination of the Helicobacter pylori infection can produce...
Reperfusion Injury of Cold-Preserved Rat Liver
M. Kopecký, P. Baláš, V. Semecký, L. Tilšer, E. Rouchalová
Čes. slov. farm. 2002, 51(2):84-90
Histological changes which appear as a result of reperfizsion injury of cold-preserved rat liver were studied at intervals of 0 hr, 3 hr, 24 hr and 48 hr of cold storage. The isolated livers were stored in a UW solution (University of Wisconsin), which is used in human liver transplantations. Computer image analysis of light microscopic sections (methyl green-pyronin stained) was used for the study and quantification of injured cells. The method of TUNEL was performed to prove possible apoptosis of sinusoidal endothelial cells and heptocytes. Bile production during reperfizsion and ALT, AST, LDH and ACP were measured in the reperfizsion medium at the...
Validation of a HPLC Method for the Determination of Dioxopromethazine and Phenylephrine in Eye Drops
T. Hudecová, Š. Hatrík, N. Zimová, E. Havránek
Čes. slov. farm. 2002, 51(2):91-95
The present paper introduces a rapid HPLC method for the determination of dioxopromethazine and phenylephrine in eye drops. The method uses a modified C18 stationary phase optimized for the separation of basic compounds and a methanoUl.5 mM phosphoric acid (60/40 v/v, pH 3.02) mobile phase. The flow rate is set to 2 mUmin, sample volume 20 pl, and compounds are detected at 275 nm. Prior to analysis, the eye drops are diluted with water in a rado of 1:50. The elaborated HPLC method and the chromatographic system esere validated according to the procedure for the validation of chromatographic systems and methds.
The Effect of Jasminic Acid on the Production of Flavonoids by the Culture Ononis arvensis L, in vitro
L. Tůmová, L. Zápalková
Čes. slov. farm. 2002, 51(2):96-98
The present paper examined the effect of jasminic acid in three different concentrations (1000 mg/1; 10 mg/1, and 0.1 mg/1) on the formation of fiavonoids in the culture Ononis arvensis L. in nitro. The tested concentrations of jasminic acid esere applied to the callus and suspension cultures of Ononis arvensis L. in the 4th week of culture growth and elicitation took place for 12; 24; 48; 72, and 168 hours. A statistically significant increase in the content of fiavonoids was found in all administered solutions of jasminic acid after 12 hours of elicitation, excepting the concentration c2 (10 mg/1) in the suspension culture. The highest yield was...
Pre-Formulation Studies of a Potential Local Anaesthetic from the Group of Phenylcarbamic Acid Derivatives
I. Procházka
Čes. slov. farm. 2002, 51(2):99-101
Within pre-formulation studies of phenylcarbamic acid derivatives with local anaesthetic effects, potential drog IV with the chemical name N-(2-(2-ethylphenylcarbamoyloxy)-ethyl)-piperidiniumchloride was examined. The study evaluated the effect of polyhydric alcohols - glycerol, propylene glycol, sorbitol and their concentrations of 5; 10; 15, and 20 wt % on the values of the partition coefficient in the system n-octanoUwater, surface tension, and viskozity of the solutions of potential drog IV. The optimal auxiliary substance seems to be glycerol of a concentration of 15-20 wt %, as it does not affect the values of the partition coefficient and surface...