JOURNAL OF THE CZECH PHARMACEUTICAL SOCIETY AND THE SLOVAK PHARMACEUTICAL SOCIETY

Čes. slov. farm. 2016, 65(4):161-165 | DOI: 10.36290/csf.2016.031

Phenolic compounds in leaves insertions of Mentha × villosa Huds. cv. Snežná

Daniela Tekeľová*, Silvia Bittner Fialová, Jaroslav Tóth, Szilvia Czigle
Univerzita Komenského v Bratislave, Farmaceutická fakulta, Katedra farmakognózie a botaniky, Bratislava, SR

Lamiaceae plants mostly accumulate active ingredients in their leaves. The subfamily Nepetoideae, including the genus Mentha L., is characterized by the presence of essential oil and antioxidant phenolics, chiefly hydroxycinnamic acids with predominance of rosmarinic acid, and flavonoids. Mentha × piperita and M. spicata are the most broadly used mints in both medicine and industry, while M. x villosa is less known in our country. Herbal drugs in the form of leaves are usually analysed unpartitioned, while single leaves insertions have only been studied occasionally. Therefore, the aim of this work was the quantification of the active compounds content in the leaves pairs of Mentha × villosa Huds. cv. Snežná, using pharmacopoeial methods: total hydroxycinnamic derivatives expressed as rosmarinic acid (THD) and luteolin-type flavonoids. THD content ranged from 6.7% to 9.4% in the leaves pairs' water extracts, and from 6.6% to 14.0% in methanol extracts. Flavonoids contents, expressed as luteolin-7-O-glucoside, ranged from 4.0% to 8.8% in water extracts, and from 4.0% to 10.5% in methanol extracts. Antioxidant activity (DPPH) expressed as SC50 ranged from 10.2 to 16.9 μg.ml-1 (drug dry weight) in water extracts, and from 10.7 to 21.6 μg.ml-1 in methanol extracts. The highest content of phenolic compounds as well as the highest antioxidant activity were found to be in the top sheet, while the lowest content of phenolic compounds and lowest antioxidant activity were detected in the leaves of the middle stem part.

Keywords: Mentha × villosa Huds cv. Snežná; hydroxycinnamic derivatives; rosmarinic acid; luteolin-7-O-glucoside; DPPH
Grants and funding:

Práca bola podporená grantovým projektom VEGA MŠ SR č. 1/0290/16 (Komplexná analýza látok prírodného pôvodu s terapeutickým potenciálom v humánnej medicíne).

Received: July 15, 2016; Accepted: August 11, 2016; Published: April 1, 2016  Show citation

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Tekeľová D, Bittner Fialová S, Tóth J, Czigle S. Phenolic compounds in leaves insertions of Mentha × villosa Huds. cv. Snežná. Čes. slov. farm. 2016;65(4):161-165. doi: 10.36290/csf.2016.031.
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