Čes. slov. farm. 2022, 71(1):27-33 | DOI: 10.5817/CSF2022-1-27

Surviving of production probiotic strains in a selected application form

Adriána Fečkaninová^1,*, Jana Koščová², Aleš Franc³, Dagmar Mudroňová², Peter Popelka⁴

¹ Univerzita veterinárskeho lekárstva a farmácie, Katedra farmaceutickej technológie, farmakognózie a botaniky, Košice, SR

² Univerzita veterinárskeho lekárstva a farmácie v Košiciach, SR, Katedra mikrobiológie a imunológie

³ Masarykova univerzita, Brno, ČR, Farmaceutická fakulta, Ústav farmaceutické technologie

⁴ Univerzita veterinárskeho lekárstva a farmácie v Košiciach, SR, Katedra hygieny, technológie a zdravotnej bezpečnosti potravín

Research in probiotics for aquaculture is at an early stage of development and much work is still needed. Lactiplantibacilli belong to the microorganisms most frequently used to prepare the probiotics. The available information is inconclusive, since few experiments with sufficiently robust design have been conducted to permit critical evaluation. The development of probiotics applicable to commercial use in aquaculture is a multistep and multidisciplinary process requiring both empirical and fundamental research, full-scale trials, and an economic assessment of its use. The aim of the study was to prepare a probiotic aquafeed via excipients and subsequently to observe the survival of probiotic bacterial cells in the feed during the nine months storage period at a refrigerator (4 °C) or room temperature (22 °C). The strain Lactobacillus plantarum R2 Biocenol™ (CCM 8674) (according to the new taxonomy Lactiplantibacillus plantarum), potentially usable as a probiotic in aquaculture, was administered to prepare the aquafeed. Better survival of probiotic bacterial cells was recorded in a samples of pellets A (Aquatex 41 HMD) compared to the samples of probiotic pellets B (Inicio 918-2). Since oxidation of fatty acids in feed affects the nutritional quality of individual feed components, we assume that higher amounts of oil in feed B negatively affected the survival of probiotic bacterial cells. The highest numbers of viable probiotic bacteria cells were recorded at 4 °C storage of probiotic feed samples. The number of lactiplantibacilli dropped from 7.30 log₁₀CFU . g^-1 to 5.57 log₁₀CFU . g^-1 after the nine months storage period of feed samples A at 4 °C. Temperature is considered as a critical factor influencing probiotic viability and survival during storage period.

Keywords: viability; excipients; coating; aquafeed; Lactiplantibacillus plantarum

Grants and funding:

Práca bola podporená z finančných prostriedkov Agentúry na podporu výskumu a vývoja na základe zmluvy č. APVV-19-0234 a Vedeckej grantovej agentúry - projekt VEGA 1/0731/21.

Received: October 25, 2021; Accepted: January 18, 2022; Published: January 1, 2022  Show citation