The inhibition activity of selected ß-carboline alkaloids on enzymes of acetylcholinesterase and butyrylcholinesterase

Zuzana Kršková, Jan Martin, Jaroslav Dušek

Čes. slov. farm. 2011, 60(3):125-131  

This thesis deals with testing of inhibition activity ß-carboline alkaloids on activity of enzymes acetylcholinesterase (ACHE) and butyrylcholinesterase (BUCHE) using test "Fast Blue B salt" at TLC desk and Ellman's test using spectrophotometer. It was also investigated how dimethylsulfoxide used as a solvent in combination with water affects activity of enzymes and alkaloids. Results show harmine in form of base and salt in water and in mixture of DMSO and water has the hightest inhibition activity on ACHE using eserine as reference substance. Harmalol in form of salt in water and harmine in form of base and salt in mixture of DMSO and water has the...

Influence of membranes on alaptide permeation from hydrogels

Zuzana Vitková, Veronika Šmunková, Petra Herdová, Marián Žabka

Čes. slov. farm. 2011, 60(3):132-136  

The paper evaluates the liberation of alaptide from gels through various types of permeable membranes. The gels were prepared on the basis of three different polymers (3% chitosan; 2.5% hydroxypropyl cellulose; 3% hydroxyethyl cellulose) in different concentrations with additions of humectants (5 %; 15% propylene glycol and 10% glycerol) and the preserving agent, 0.3% Sepicide HBR with 1% alaptide, and finally without the drug. The permeation of the drug from gels into the acceptor solution was evaluated with the use of the following membranes: the hydrophilic membrane from Chemosvit, the chicken skin, the stripped snakeskin, and the wall of the small...

Synthesis and basic physicochemical properties of 1-[3-(Y-alkoxyphenylcarbamoyloxy)-2--hydroxypropyl]-4-(2-methylphenyl)piperazinium chlorides

Ivan Malík, Eva Sedlárová, Fils Andriamainty, Jana Gališinová, Jozef Csöllei, Ján Vančo

Čes. slov. farm. 2011, 60(3):137-143  

1-[3-(Y-Alkoxyphenylcarbamoyloxy)-2-hydroxypropyl]-4-(2-methylphenyl)piperazinium chlorides (labelled as 7a-7d) were prepared within a complex study of the relationships between the chemical structure, physicochemical properties and biological (antiarrhythmic, antihypertensive) activity of dual acting compounds. Chemical structures of basic forms (labelled as 7a2C-7d2C) and appropriate monochlorides (labelled as 7a-7d) were confirmed by ¹H-NMR, ¹³C-NMR, MS and IR spectral and elemental analysis. The estimated physicochemical parameters included the melting point data, solubility profile in various media,...

Effects of health-service reform in the field of medicinal drugs. I. Analysis from the standpoint of a pharmacy

Božena Macešková, Jana Streitová

Čes. slov. farm. 2011, 60(3):99-104  

The paper examines the effects of changes in medicinal drug policy valid since 1 January 2008 by comparing the dispensation to patients in a pharmacy supplying a hospital in the years 2007 and 2008. The examination was performed using financial representation (sale price without VAT), the number of original packages and classification according to ATC groups. The data were obtained from the information system of the pharmacy. In the year 2008 the total number of received prescriptions decreased by 29.05% versus the year 2007, the payments from health insurance companies decreased by 12.85%, the total number of dispensed packages decreased by 18.20%,...

Effects of health-service reform in the field of medicinal drugs. II. Analysis from the standpoint of financial participation of the patient in pharmacotherapy

Božena Macešková, Miroslav Koška

Čes. slov. farm. 2011, 60(3):105-109  

Since 1 January 2008, the health service reform established the duty to pay a regulatory fee for the dispensation of one item on the prescription which is at least partially covered by the public health insurance and it also established a protective annual limit of the sum of a patient's financial participation in the provided health care. The study aimed to determine the effects of these measures. The complete medication of 100 patients for the year 2008 was examined. The patients' prescriptions included 2 062 items, out of which 841 items (41 %) were fully covered. The share of the items with non-zero supplementary charge was about 20 %. In the year...

Clinical significance of cytochrome P450 genetic polymorphism - Part I. Enzymatic system of cytochrome P450 and cytochrome P450 1A2

Jana Ďuricová, Milan Grundmann

Čes. slov. farm. 2011, 60(3):110-115  

Inter-individual variability in drug response is a major clinical problem. Much of the variability has been observed in drug metabolism, particularly in the enzymes of cytochrome P450. Genetic polymorphism in these enzymes may influence a patient's response to commonly prescribed drugs. The first part of this review describes the enzymatic system of cytochrome P450 and further focuses on the influence of genetic polymorphism of cytochrome P450 1A2 on drug effect.

Farmaceutické aspekty živočíšnej lipoxygenázy

Marek Obložinský, Lýdia Bezáková, Renáta Kollárová

Čes. slov. farm. 2011, 60(3):116-124  

Lipoxygenases (LOX, plant LOX [EC 1.13.11.12], linoleate: oxygen oxidoreductase, animal LOXs [5-LOX, EC 1.13.11.34; 8-LOX, EC 1.13.11.40; 12-LOX, EC 1.13.11.31; 15-LOX, EC 1.13.11.33], arachidonate: oxygen oxidoreductase) belong to the family of structurally related dioxygenases containing non-heme and non-sulfide iron in the active site. LOX catalyzes the regiospecific and stereospecific insertion of molecular oxygen into the molecule of unsaturated fatty acid with the (1Z,4Z)-penta-1,4-diene structural unit in its aliphatic chain. The result of this reaction is the production of conjugated optically active (S)- or (R)-hydroperoxides...

Index Nominum. International Drug Directory Vol. 1 a Vol 2. 20th edition.

J. Čižmárik

Čes. slov. farm. 2011, 60(3):131  

Lacinová, Ľ., Uhríková, D.: Biofyzika napäťovo závislých iónových kanálov

Jozef Čižmárik

Čes. slov. farm. 2011, 60(3):136  

XXXIII. pracovní dny sekce radiofarmacie České společnosti nukleární medicíny ČLS JEP. Rožnov pod Radhoštěm, 1.-3. června 2011

Čes. slov. farm. 2011, 60(3):144-152  

Zprávy

Čes. slov. farm. 2011, 60(3):153-154