JOURNAL OF THE CZECH PHARMACEUTICAL SOCIETY AND THE SLOVAK PHARMACEUTICAL SOCIETY

Čes. slov. farm. 2011, 60(3):125-131

The inhibition activity of selected ß-carboline alkaloids on enzymes of acetylcholinesterase and butyrylcholinesterase

Zuzana Kršková, Jan Martin*, Jaroslav Dušek
Department of Pharmacognosy, Charles University in Prague, Faculty of Pharmacy in Hradec Králové, Czech Republic

This thesis deals with testing of inhibition activity ß-carboline alkaloids on activity of enzymes acetylcholinesterase (ACHE) and butyrylcholinesterase (BUCHE) using test "Fast Blue B salt" at TLC desk and Ellman's test using spectrophotometer. It was also investigated how dimethylsulfoxide used as a solvent in combination with water affects activity of enzymes and alkaloids. Results show harmine in form of base and salt in water and in mixture of DMSO and water has the hightest inhibition activity on ACHE using eserine as reference substance. Harmalol in form of salt in water and harmine in form of base and salt in mixture of DMSO and water has the hightest activity on BUCHE. It was find out that DMSO considerably affects activity of enzymes and alkaloids.

Keywords: ß-carboline alkaloids; ACHE; BUCHE; DMSO; Ellmanęs test; test "Fast Blue B salt"
Grants and funding:

This study was supported by the Charles University in Prague (Project SVV 2010-261-002) and by MSM 0021620822 research project.

Received: April 4, 2011; Accepted: May 6, 2011; Published: March 1, 2011  Show citation

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Kršková Z, Martin J, Dušek J. The inhibition activity of selected ß-carboline alkaloids on enzymes of acetylcholinesterase and butyrylcholinesterase. Čes. slov. farm. 2011;60(3):125-131.
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