Czech and Slovak Pharmacy, 2000 (vol. 49), issue 2
Articles
Mucoadhesive Oral Tablets: A Modern Dosage Form with Controlled Release of the Active Ingredient
M. Dostálová, M. Rabišková
Čes. slov. farm. 2000, 49(2):55-61
The review paper summarizes the principal pharmaceutical and medical information about thehitherto achieved results in the development of bioadhesive dosage form concentrating on mucoad-hesive oral tablets. The site of administration of the tablets is the facial pocket. Due to specificproperties of the mucous layer on the buccal mucosa and mucoadhesive polymers from which thetablets are manufactured, tablets adhere to the mucosa. The adhesion may take a certain period oftime and during the whole period the active ingredient is gradually released. Absorption of the drugthrough the mucous membrane of the oral cavity has a number of advantages, in particular...
Effective and Safe Pharmacotherapy of Acne vulgaris and the Treatment of SolarImpairment of the Skin
Z. Fendrich, E. Jandová, M. Finsterlová
Čes. slov. farm. 2000, 49(2):62-67
An inevitable condition for the pharmacist is a basic knowledge of dermatological changes whichare prominent in acne and solar impairment of the skin to be able to recommend in a qualifiedmanner an effective and safe treatment to the patient. However, sufferers of the more serious forms of acne should always be referred to their general practitioner, or preferentially a dermatologist. Acne vulgaris is an androgen-induced disorder, but three major mechanisms for the development of the disease have been identified: hypertrophy of the sebaceous gland, hyperkatosis of the follicularepithelium, and proliferation of microbial flora, particularly Propionibacterium...
Chemical Structure of New Quinolones and Their Antibacterial ActivityII. 4 Quinolones: Their Natural Occurrence and Biological Properties
V. Milata
Čes. slov. farm. 2000, 49(2):68-74
The review paper deals with the natural occurrence, biological properties, and uses o f 4-quinolonesconcentrating on 3-substituted-4-quinolones and their antimicrobial activity.
Apigenin-7-methylglucuronide from Cynara cardunculus L.
P. Mučaji, D. Grančai, M. Nagy, Z. Višňovská, K. Ubik
Čes. slov. farm. 2000, 49(2):75-77
From an ethanolic extract of the flower buds of Cynara cardunculus L. (Asteraceae), apigenin-7--methylglucuronide and chlorogenic acid were isolated. The isolated compounds were identified byspectroscopic means, by comparison with authentic samples and literature data.
Effect of Linoleic Acid on the Production of Secondary Metabolites
L. Tůmová, J. Dušek
Čes. slov. farm. 2000, 49(2):78-81
The paper examines the effect of linoleic acid in various concentrations on the production offlavonoids in an Ononis arvensis culture in vitro and the production of anthracene derivatives ina Rheum palmatum culture in vitro. The content of the metabolites under study after elicitationwith linoleic acid, which is the precursor of jasminic acid intervening into the course of elicitation,was examined after 12; 24; 48 and 168 hours. The maximal increase in the content of flavonoids(118 %) was found after the application of linoleic acid in a concentration of 2 mg/ml after 48 hrs.In concentrations of 0.01; 0.20, and 1 mg/ml after 48 hours of application...
HPLC Analysis of Non-Steroidal Antirheumatics in Biological Material IV. Acetic Acid Derivatives
J. Sochor, J. Klimeš, J. Sedláček, P. Čermáková
Čes. slov. farm. 2000, 49(2):82-87
The present paper surveys the published HPLC methods estimating derivatives of acetic acid inbiological materials and an HPLC method for estimation of indomethacine in the samples of wholeblood. The elaborated chromatographic conditions and process of isolation were applied also to theanalysis of tropesine and both drugs were determined simultaneously. The blood samples wereadjusted by liquid-liquid extraction and indomethacine (and tropesine) was analysed on the reversephase Separon SGX C-18 with the mobile phase methanol - water (pH 3.2) and detected at 254 nm.The method was used in a pharmacokinetic study of indomethacine on rabbits.
Determination of Xanthinol Nicotinate in Dosage Forms by Capillary Isotachophoresis
M. Pospíšilová, V. Jokl, J. Dolejšová
Čes. slov. farm. 2000, 49(2):88-90
Cationic capillary isotachophoresis (ITP) with coductimetric detection has been used for separating and determination of xanthinol in two commercial mass-produced preparations. The optimized ITP electrolyte system consisted of 5 mM potassium picolinate + 5 mM picolinic acid (pH 5.21) as the leading electrolyte and 10 mM formic acid as the terminating electrolyte. The driving and detection currents were 50 mA (for 380 s) and 10 mA, respectively. The analysis took about 8 min. According to the validation procedure based on the reference UV spectrophotometric method, the ITP method gave practically the same results.
99mTc-Labelled Product of Photochemical Degradation of Insulin
I. Kleisner, P. Komárek, J. Klančík, M. Konopková, I. Komárková
Čes. slov. farm. 2000, 49(2):91-95
Irradiation with UV light at a wavelength of 250 nm allows to convert disulphidic groups in thestructure of insulin to free SH groups with high affinity to direct labelling with 99mTc. The amountof terminal SH groups depends on irradiation time. HPLC analysis revealed that while 34% of theoriginal amount of insulin was present after 15 minutes of irradiation (approx. intensity of500 mW.cm2), the amounts were 25%, 10%, and 2% after 30; 60, and 120 minutes, respectively. Theseresults correlate with the amount of SH groups released by photoactivation measured using Ellman'sreagent. The product of the degraded porcine insulin was labelled with...
Pharmaceutical Dosage Forms as the Subject of a Dissertation in the Historic Past of Charles University
H. Žáček, V. Rusek
Čes. slov. farm. 2000, 49(2):96-99
The "galenical" dissertation which was presented for disputation by Georgius Horčička at Charles University in 1841 is discussed as far as its content is concerned. The dissertation studies a great number of pharmaceutical dosage forms, many of which are already obsolete, but a certain number of which are still in use in the present time. It is probably one of the first dissertations treating of the given subject.