Czech and Slovak Pharmacy, 1999 (vol. 48), issue 1
Articles
Antioxidants of Biogenic Origin
R. Kubínová, V. Suchý
Čes. slov. farm. 1999, 48(1):9-14
Diseases due to free radical are the subject of intensive study. The search aims to find substances preventing the origin of free radicals, substances capable of rendering the developed free radicals harmless, or substances capable of removing the damage of molecules which has already taken place. The present paper briefly surveys the metabolites of biogenic origin, mainly from higher plants, which show in vitro antioxidant activity and are potentially usable in a number of diseases.
Kinetics of Hydration of Lyophilised Xerogel of Hydroxypropylmethylcellulose
Z. Zatloukal
Čes. slov. farm. 1999, 48(1):15-17
The rate of hydration of lyophilised ophthalmic lamellae of graded density of 50 to 56g/l in water or an aqueous solution of sodium chloride was estimated by means of ahydrophilic filtration membrane. With insignificant effect of density, the non-linearcourse of hydration is affected mainly by the diffusion mechanism of penetration of waterinto xerogel while preserving its shape in a time interval of to 20 minutes. In a periodof 10 minutes the rate of hydration of 19.9 mm/min isdecelerated to 15.3 mm/min in the presence of sodium chloride.This contributes to the elucidation of different rates of the release of activeingredients from ophthalmic lamellae...
Calorimetric Determination of Silicone Matrices
M. Řehula, M. Musilová, J. Lavická, V. Velich, M. Kroupa
Čes. slov. farm. 1999, 48(1):18-20
The present paper investigates the effects of particle size and the amount of sodiumchloride on specific dissolving heat of silicone matrices. Specific dissolving temperaturewas measured on a calorimeter with a heat flow SETARAM C 80. Silicone matrices containing15% and 30% of sodium chloride with particle sizes 20 mm, 450 mm and 755 mm were evaluated. With growingparticle size from 20 mm to 755 mmthe values of the specific dissolving heat were increased 2.3 times; with the increasingamount of sodium chloride in the silicone matrix from 15% to 30% the values of thespecific dissolving heat were increased 1.8 times. A linear relationship was found betweenthe...
A Study of Labeling Conditions of Derivatives of (2RS, 4R)-1,3-Carboxylic Acid with Technetium-99m
A. Bumbálová, E. Havránek, J. Ja'afreh, V. Kettmann, J. Světlík, O. Štroffeková
Čes. slov. farm. 1999, 48(1):21-26
The preparation of 99mTc-complexes with twenty derivatives of (2RS,4R)-1,3-thiazolidin-4-carboxylic acid over a broad pH range (1.5-8.9) is described for a potential use in radiodiagnostic medicine. The labelling efficiency was checked by paper and thin layer chromatography and for all well-soluble compounds is higher than 90 %. Paper electrophoresis and gel chromatography indicated the presence of two types of complexes differing in charge and molecular mass. The structure of both types of the 99mTc-complexes is also discussed. The organ distribution and its time dependence were determined in mice after i.v. application.
Targeting and Controlled Release of Drugs Using Magnetoliposomes
M. Babincová
Čes. slov. farm. 1999, 48(1):27-29
The present paper deals with a stable drug delivery system which allows to concentrate the drug in a desired site and its programmable release. For these purposes the present authors have suggested to use magnetoliposomes (liposomes with enwrapped magnetite particles in their bilayers) which are magnetosensitive and may be maneuvered to the given site in the organism. Due to the magnetite particles, which are strong microwave absorbers, magnetoliposomes can be heated to higher temperatures which may subsequently lead to a leakage of encapsulated drug. Influence of static magnetic field on liposomes in the blood-stream was analyzed theoretically. Fluorescence...
Invertase in Immobilized Poppy Cells
J. Stano, P. Kovács, K. Neubert
Čes. slov. farm. 1999, 48(1):30-33
Cell suspension culture of the opium poppy plant (Papaver somniferum L.) was permeabilized by Tween 80 and immobilized by glutaraldehyde. The pH optimum of the immobilized cells' invertase is 4.6 and the temperature optimum is 50 °C. The enzyme hydrolysis was linear for 5 h, reaching 69 % of conversion. The immobilized cells are characterized by a high invertase activity and showed convenient mechanical properties.
Microscopic Description of the Stem and Leaves of Stevia rebaudiana (Bertoni) HEMSLEY - (Asteraceae)
J. Spilková, H. Holečková
Čes. slov. farm. 1999, 48(1):33-35
The paper presents a microscopic description of Steviae herba supplemented with pictures. Multicellular covering trichomes and spherical glands occurring in the leaves are important for identification of the drug.
Effect of the Elicitor from Rhodotorula rubra on the Production of Flavonoids in the Culture of Ononis arvensis L. in vitro
L. Tůmová, K. Babáková
Čes. slov. farm. 1999, 48(1):36-39
The effect of the elicitor - a solution of a homogenate and killed cells of Rhodotorula rubra in three different concentrations on the production of flavonoids in a callus and suspension culture of Ononis arvensis L. was tested. The elicitor was in contact with the culture for 24; 48 and 168 hours. It follows from the obtained results that in the case of both the homogenate and the solution of the cells of Rhodotorula rubra there occurred a significant increase in the production of flavonoids with the use of the concentration c3 after 48 hours' elicitation. However, even after 24 hours of elicitation the content of flavonoids was increased.
Inter-organ Variability of Enzymatic Hydrolysis of Organic Ammonium Salts in the Rat in vitro
E. Kissová, I. Paulíková, O. Helia, P. Křenek, F. Devínsky, I. Lacko
Čes. slov. farm. 1999, 48(1):40-43
The study evaluated the rate and kinetics of enzymatic hydrolysis of N-(2-benzoyloxyethyl)-alkyldimethylammonium bromides, potential easily biodegradable disinfectants of soft character. The products of enzymatic hydrolysis of the substrates under study, catalysed by microsomal esterase, included substituted substrates choline and benzoic acid which, as a hydrolytic product, was essayed by HPLC. The effect of the length of the alkyl chain of the individual homologues on the rate of enzymatic hydrolysis and their affinity to microsomal esterase of the rat liver and lung in vitro was examined. The structural modification (varying length of the aliphatic...
A Comparison of the Effect of the New Asymmetric Bispyridinium Oxime BI-6 with Oxime HI-6 and Obidoxime in Combination with Atropine on the Toxicity of Soman and Fosdrin in Mice
J. Kassa
Čes. slov. farm. 1999, 48(1):44-47
The therapeutic efficacy of the new asymmetric bispyridinium oxime BI-6 against acute toxicity of the highly toxic organophosphate soman and the organophosphorus insecticide fosdrin by means of affecting the LD50 values of these noxiores substances was compared with the effect of the hitherto most perspective oxime HI-6 and the classic obidoxime always in combination with the identical dose of atropine. At the equimolar level the effect of oxime BI-6 against fosdrin completely equals the effects of both oximes HI-6 and obidoxime. The effect of oxime BI-6 against soman is even more marked than the effect of HI-6 but this difference is not statistically...