Čes. slov. farm. 2020, 69(5):237-243

Determination of thiamine and pyridoxine in food supplements and beverages by the simple capillary zone electrophoresis in combination with UV detection

Michaela Matušková¹, Ivana Čižmárová¹, Peter Mikuš², Juraj Piešťanský^2,*

¹ Univerzita Komenského, Farmaceutická fakulta, Katedra farmaceutickej analýzy a nukleárnej farmácie

² Toxikologické a antidopingové centrum, Bratislava, Slovenská republika

The paper is focused on development of a simple analytical method based on capillary zone electro-phoresis in combination with UV-detection for simul-taneous detemination of thiamine and pyridoxine in pharmaceutical and food samples. The separation of thiamine and pyridoxine was performed in a background electrolyte composed of 25 mmol ∙ l^-1 GABA + 50 mmol ∙ l^-1 HAc+ 0.05% m-HEC. The UV detector was set at the constant wavelength of 260 nm. Limit of detection was 0.059 µg ∙ ml^-1 for thiamine and 0.23 µg ∙ ml^-1 for pyridoxine. These levels suggest that relatively low quantities of thiamine and pyridoxine can be detected. The presented CZE-UV method enabled effective determination of the two vitamins in 5 food supplements and 11 energy drinks and vitamin waters.

Keywords: Thiamine; Pyridoxine; capillary zone electrophoresis; UV detection; quality control

Grants and funding:

Práca vznikla s podporou grantu APVV-15-0585, VEGA 1/0463/18, KEGA 027UK-4/2020.

Received: November 10, 2020; Accepted: December 8, 2020; Published: May 1, 2020  Show citation