JOURNAL OF THE CZECH PHARMACEUTICAL SOCIETY AND THE SLOVAK PHARMACEUTICAL SOCIETY

Čes. slov. farm. 2018, 67(1):25-29 | DOI: 10.36290/csf.2018.003

The immunomodulatory activity of the extracts and complexes of biologically active compounds of Galium verum L. herb

Igor L. Shinkovenko1, Natalia V. Kashpur2, Tatiana V. Ilyina1, Alla M. Kovalyova1, Olga V. Goryacha1,*, Oleh M. Koshovyi1, Erica L. Toryanyk1, Olena V. Kryvoruchko1
1 National University of Pharmacy, Kharkiv, Ukraine
2 SO Mechnikov Institute of Microbiology and Immunology, Kharkiv, Ukraine

The present article discusses the results of an immunomodulatory activity of Galii veri herba, Galium verum herb (Galium verum L., Rubiaceae) fluid water extract and the effect of the polysaccharide and phenolic complexes of the letter on the immunomodulatory activity. The same extract was fractionated into a polysaccharide complex (PSC) and a polyphenolic complex (PPC). In the obtained substances, the contents of hydroxycinnamic derivates, flavonoids and polyphenols were determined spectrophotometrically; polysaccharides were quantified gravimetrically; the immunomodulatory activity of the substances was determined in the reaction of lymphocyte blast transformation. It has been established that the fluid extract of Galium verum herb contains 6.3% polysaccharides, 4.2% hydroxycinnamic derivates expressed as chlorogenic acid, 0.4% flavonoids expressed as rutin, 3.7% polyphenols expressed as gallic acid. PPC contains 4.48% hydroxycinnamic derivates expressed as chlorogenic acid, 0.43% flavonoids expressed as rutin, and 3.95% polyphenols expressed as gallic acid. The lowest immunomodulatory activity was found for PPC. A significantly higher activity was determined for PSC. The highest immunomodulatory activity was established for the fluid extract at a dilution of 1/20, its activity being by 59.4% higher compared with the lymphocyte spontaneous transformation and by 18.5% higher than that of the reference substance PHA. The obtained results enable an assumption of a synergistic effect of PPC and PSC of Galium verum herb fluid extract on the potency of its immunomodulatory activity.

Keywords: Galium verum L.; immunomodulatory activity; lymphocyte blast transformation; fluid water extract; polysaccharide complex; polyphenols complex
Grants and funding:

This study was supported by the Laboratory of Immunorehabilitology of the Mechnikov Institute of Microbiology and Immunology of National Academy of Sciences of Ukraine.

Received: November 5, 2017; Accepted: January 6, 2018; Published: January 1, 2018  Show citation

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Shinkovenko IL, Kashpur NV, Ilyina TV, Kovalyova AM, Goryacha OV, Koshovyi OM, et al.. The immunomodulatory activity of the extracts and complexes of biologically active compounds of Galium verum L. herb. Čes. slov. farm. 2018;67(1):25-29. doi: 10.36290/csf.2018.003.
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    References

    1. Chinen J., Shearer W. Secondary Immunodeficiencies, including HIV infection. Journal of Allergy and Clinical Immunology 2010; 125(2), 195-203. Go to original source... Go to PubMed...
    2. Xiubao C., Yuanxiao Z., Changxin S., Stewart A.K. Mechanism of immunomodulatory drugs' action in the treatment of multiple myeloma. Acta Biochim. Biophys. Sin. (Shanghai) 2014; 46(3), 240-253. Go to original source... Go to PubMed...
    3. Alamgir M., Uddin S. J. Recent advances on the ethnomedicinal plants as immunomodulatory agents. Ethnomedicine: A Source of Complementary Therapeutics 2010; 4, 227-244.
    4. Uorakkottil I., Deepshikha P. K., Vidhu A., Punnooth P. N. A Review on Hepatoprotective and Immunomodulatory Herbal Plants. Pharmacogn Rev. 2016; 10(19), 66-70. Go to original source... Go to PubMed...
    5. Pushpa R., Nishant R., Kumar N., Gautam P. Antiviral potential of medicinal plants: an overview. Int. Res. J. Pharm. 2013; 4(6), 8-16. Go to original source...
    6. Silva N. C. C., Fernandes Jr A. Biological properties of medicinal plants: a review of their antimicrobial activity. J. Venom. Anim. Toxins incl. Trop. Dis. 2010; 16(3), 402-413. Go to original source...
    7. Ambriz-Pérez D. L., Leyva-López N., Gutierrez-Grijalva E. P., Heredia J. B. Phenolic compounds: Natural alternative in inflammation treatment. A review. Cogent Food & Agriculture. 2016; 2(1): 1131412. Go to original source...
    8. Mohamed S. I. A., Jantan I., Haque M.A. Naturally occurring immunomodulators with antitumor activity: An insight on their mechanisms of action. Int. Immunopharmacol. 2017; 50, 291-304. Go to original source... Go to PubMed...
    9. Kure Ch., Timmer J., Stough C. The immunomodulatory effects of plant extracts and plant secondary metabolites on chronic neuroinflammation and cognitive aging: a mechanistic and Empirical Review. Front Pharmacol. 2017; 8, 117. Go to original source... Go to PubMed...
    10. Abubakirov N. K., Belenovskaya L. M., Grushvitskaya I. V., et al. Plant resources of the USSR: Flowering plants, their chemical composition and use; Families Caprifoliaceae-Plantaginaceae, Leningrad: Nauka 1990, 326 p. (in Russian)
    11. The State Pharmacopoeia of Ukraine / State enterprise "Scientific and Expert Pharmacopoeial Centre", First edition, Kharkiv: RІREG, 2001; 556 p. (in Ukrainian).
    12. The State Pharmacopoeia of Ukraine / State enterprise "Scientific and Expert Pharmacopoeial Centre", First edition, first supplement, Kharkiv: RІREG, 2004; 494 p. (in Ukrainian).
    13. Minin S. A., Kauhova E. I. Chemistry & Technologies of phytopreparations. Moscow: Heotar - Honey, 2004, 516 p. (in Russian).
    14. Yezerska O., Kalynyuk T., Vronska L. Quantitative determination of hydroxycinnamic acids in Chicory root. Chemistry and Chemical Technology 2013; 7(3), 247-250. Go to original source...
    15. Spagnol C. M., Oliveira Th. S., Lucia Borges V. I., Corrêa M. A., Salgado H. R. N. Validation of caffeic acid in emulsion by UV-Spectrophotometric method. Physical Chemistry 2015; 5(1), 16-22.
    16. The State Pharmacopoeia of Ukraine / State enterprise "Scientific and Expert Pharmacopoeial Centre", First edition, second supplement, Kharkiv: RІREG, 2008; 617 p. (in Ukrainian).
    17. Kovalyova A. A., Georgievskiy G. V., Kovalyov V. M., Komisarenko A. M. et al. Development of new piflamin medicine standardization methods. Farmakom 2002; 2, 92 -97 (in Ukrainian).
    18. Koshovyi O. M., Zagayko A. L., Kolychev I. A., Akhmedov E. Yu., et al. Phytochemical study of the dry extract from bilberry leaves. Azerbaijan Pharmaceutical and Pharmacotherapy Journal 2016; 1, 18-23 (in Russian).
    19. Korneeva M. N., Novokhatskii A. S., Grebenyuk V. N., Kerimov S. G. Use of the lymphocyte blast transformation reaction to assess the state of cellular immunity. Bulletin of Experimental Biology and Medicine 1989; 107(4), 533-535. Go to original source...
    20. Bashirova D. K., Kochnev O. S., Davletkil'deev F. A., Lagutina M. V. Immunologic activity of human lymph cells in the lymphocyte blast transformation reaction. Biull. Eksp. Biol. Med. 1980; 89(1), 33-35. Go to original source...
    21. Bulanova E. G., Budagyan V. M., Yarilin A. A., Mazurenko N. N. Expression of protooncogenes during lymphocyte activation by growth factors. http://protein.bio.msu.ru/biokhimiya/contents/v62/full/62091191.html
    22. Movafagh A., Heydary Н., Mortazavi-Tabatabaei S. A., Azargashb E. The significance application of indigenous phytohemagglutinin (PHA) mitogen on metaphase and cell culture procedure. Iran. J. Pharm. Res. 2011; 10 (4), 895-903. Go to original source... Go to PubMed...
    23. The State Pharmacopoeia of Ukraine / State enterprise "Scientific and Expert Pharmacopoeial Centre", First edition, fourth supplement, Kharkiv: RІREG, 2011; 538 p. (in Ukrainian).
    24. Zulfiqar A., Bhaskar S. B. Basic statistical tools in research and data analysis. Indian J Anaesth. 2016; 60(9), 662-669. Go to original source... Go to PubMed...
    25. Zhao C., Shao J., Cao D., Zhang Y., Li X. Chemical constituents of Galium verum. Zhongguo Zhong Yao Za Zhi 2009; 34(21), 2761-2764.
    26. Vlase L., Mocan A., Hanganu D., Benedec D., Gheldiu A., Crișan G. Comparative study of polyphenolic content, antioxidant and antimicrobial activity of four Galium species (Rubiaceae). Digest Journal of Nanomaterials and Biostructures 2014; 9(3), 1085-1094.
    27. Ghiţă G., Necula R., Trifan A., Gille E., Zamfirache M. M., Stănescu U. Investigations regarding the phytochemical study of some samples of Galium verum L. and Galium album Mill. Analele Ştiinţifice ale Universităţii "Al. I. Cuza" Iaşi s. II a. Biologie vegetală. 2012; 58(1), 45-50.
    28. Kashpur N. V., Yurchenko N. S., Ilyina T. V., Kovalyova A. M., Goryacha О. V., Smilyanska M. V., Peremot S. D. The immunomodulatory effect of Asperula odorata L. and Asperula humifusa M. Bieb. Besser dry extracts. Clinical Pharmacy 2015; 19(1), 56-58. Go to original source...
    29. Amirghofran Z., Javidnia K., Bahmani M., Azadmehr A., Esmaeilbeig M. The effect of the methanol extract of Galium mite on the cellular immunity and antibody synthesis. Journal of Immunoassay and Immunochemistry 2011; 32, 157-169. Go to original source... Go to PubMed...

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