Čes. slov. farm. 2010, 59(3):117-122

Pyrazinecarboxylic acid derivatives as effective abiotic elicitors of isoflavonoids production

L. Tůmová^1,*, J. Tůma ORCID...², M. Doležal³, B. Danielová¹

¹ Univerzita Karlova v Praze, Farmaceutická fakulta v Hradci Králové, Katedra farmakognosie

² Univerzita Hradec Králové, Pedagogická fakulta, Katedra biologie

³ Univerzita Karlova v Praze, Farmaceutická fakulta v Hradci Králové, Katedra farmaceutické chemie a analýzy léčiv

Genista tinctoria (Dyer's Greenweed) is a plant containing isoflavonoids, which rank among important estrogens. The in vitro culture of this plant produces more isoflavonoids than the parent plant. The paper aimed to even more increase the production of these substances in the callus culture by the action of abiotic elicitors, in this case pyrazinecarboxylic acid derivatives. A more suitable elicitor for the production of isoflavonoids proved to be substance A (N-(3-iodo-4-methylphenyl)pyrazine-2-carboxamide), even though the absolute maximum of genistin production was achieved by a 12-hour action of substance B (5-terc-butyl-6-chloro-N-(3-iodo-4-methylphenyl)pyrazine-2-carboxamide) in a concentration of 2.33 . 10-3 mol/l (57 times versus control). By means of elicitation using substance B, more balanced results were achieved in an increased production of isoflavonoids in the cross-section of the individual withdrawal times and concentrations.

Keywords: Genista tinctoria; isoflavonoids; callus culture; pyrazinecarboxylic acid derivatives

Grants and funding:

Práce byla vypracována za finanční podpory Výzkumného záměru MSM 0021620822.

Received: April 14, 2010; Accepted: May 15, 2010; Published: March 1, 2010  Show citation