Čes. slov. farm. 2007, 56(3):107-113

HPLC separation of enantiomers using chiral stationary phases

D. Meričko¹, J. Lehotay¹, J. Čižmárik²

¹ Faculty of Chemical and Food Technology, Slovak University of Technology Bratislava, Institute of Analytical Chemistry, Slovak Republic

² Faculty of Pharmacy, Comenius University Bratislava, Department of Pharmaceutical Chemistry Slovak Republic

During recent few years, separations of enantiomers have become one of the most important analytical tasks. Modern separation techniques, such as the chromatographic ones, represent a very powerful tool in this respect and nowadays they are commercially and widely used all over the world. The importance of enantioseparation must be emphasized, not just because of drugs, food additives, and pesticides, but also because of other compounds which affect our life. The use of direct chromatographic enantioseparation seems to be a suitable approach not just in the analytical but also the preparative scale. This paper is focused on the separation of enantiomers using the chiral stationary phase as one of the ways of how to perform direct chromatographic separation.

Keywords: HPLC; chiral separation; enantiomers; chiral stationary phases; optically active compounds

Received: February 12, 2007; Accepted: May 22, 2007; Published: March 1, 2007  Show citation