JOURNAL OF THE CZECH PHARMACEUTICAL SOCIETY AND THE SLOVAK PHARMACEUTICAL SOCIETY

Čes. slov. farm. 2006, 55(1):44-47

Explant Culture of Trifolium pratense L.

M. Kašparová, T. Siatka, J. Spilková, J. Dušek
Farmaceutická fakulta Univerzity Karlovy, Hradec Králové, Katedra farmakognozie

The red cloverl seems to be a prospective source of flavonoids and isoflavonoids with phytoestrogenic activity. Young seedlings of four different varieties of Trifolium pratense L. were employed to derive callus and suspension cultures. The optimal medium for cultivation is Gamborg's cultivating medium with an addition of 2 mg.l-1 of 2,4-dichlorphenoxyacetic acid and 2 mg.l-1 of 6-benzylaminopurine. On the basis of growth and production characteristics, subcultivating intervals of 29 to 43 days in callus culture and 16 to 23 days in suspension culture were determined. TLC and HPLC revealed that the explant culture of Trifolium pratense L. contains the isoflavonoid formononetin.

Keywords: Trifolium pratense L.; derivation of callus and suspension cultures; growth and production characteristics; flavonoids and isoflavonoids

Received: August 3, 2005; Accepted: October 11, 2005; Published: January 1, 2006  Show citation

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Kašparová M, Siatka T, Spilková J, Dušek J. Explant Culture of Trifolium pratense L. Čes. slov. farm. 2006;55(1):44-47.
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