Čes. slov. farm. 2004, 53(3):141-144

Use of Phenylglyoxylic Ketyl Radicals on the Testing of the Antioxidant Effect of VariousCompounds

F. Šeršeň¹, T. Vencel¹, A. Gáplovský¹, T. Anus²

¹ Chemický ústav Prírodovedeckej fakulty Univerzity Komenského, Bratislava

² Katedra chemickej fyziky Fakulty chemickej a potravinárskej technológie Slovenskej technickej univerzity, Bratislava

The paper describes spontaneous decomposition of D,L-2,3-diphenyltartaric acid in propane-2-ol,which takes place via radicals at ambient temperature. The primary product of the decompositionis short-living ketyl radicals of phenylglyoxalic acid, which are captured and stabilized by means ofthe spin trap of N-terc-butyl-α-phenylnitrone. The spontaneous decomposition of D,L-2,3-diphenyltartaricacid in propane-2-ol served as the source of radicals for the determination of the antioxidativeeffect of known antioxidants: C-vitamin, E-vitamin, and selenomethionine. It was found thatthe above-mentioned antioxidants are effective scavengers of radicals of phenylglyoxalic acid. Onthe basis of these data, the method can be recommended for testing the antioxidative properties ofother substances as potential antioxidants.

Keywords: C-vitamin; D,L-selenometionine; electron paramagnetic resonance; E-vitamin; spin traping; free radicals

Published: March 1, 2004  Show citation