Čes. slov. farm. 2004, 53(1):42-46
Analytical Evaluation of Potential Nootropic Agents
- Ústav chemických léčiv Farmaceutické fakulty Veterinární a farmaceutické univerzity, Brno
The paper deals with analytical evaluation of newly prepared substances, derivatives of N-(4-alkoxy-phenyl)-2-(2-oxo-azepan-1-yl)-acetamide. The substances are a homological series (methyl- to hexyl-). The purity of the substances was verified by thin-layer adsorption chromatography, and theprincipal physical characteristics - melting point and solubility - were determined. Experimentaldetermination of the partition coefficient, extraction of the substances between two liquids miscibleto a limited degree (n-octanol - water), determination of RM values by means of TLC partitionchromatography (glass plates DC-Fertigplatten® RP-8 F254S), determination of the capacity factor by means of HPLC (column C18 Plaris®), and calculation by means of computer programmes were employed to determine the lipophilicity of this series of substances. The antiradical activity of thesubstances was evaluated by the method of extinguishing the stable radical 2,2-diphenyl-1-picrylhydrazyl.Ascorbic acid, in which an antiradical effect had been demonstrated,was used for the sake of comparison. The substances show a certain activity, but they do not reach the antioxidative effectof ascorbic acid.
Keywords: nootropic agents; partition coefficient; TLC; HPLC; DPPH
Published: January 1, 2004 Show citation