Čes. slov. farm. 2003, 52(3):136-140

TLC and HPLC Separation of Positional Isomers of Alkylesters of 2-, 3-, 4- {2-Hydroxy-3--[(4-diphenylmethyl)piperazine-1-yl]propoxy}phenylcarbamic Acid and Their Lipo-HydrophilicProperties

R. Opatřilová, M. Blešová, J. Csöllei

Ústav chemických léčiv Farmaceutické fakulty Veterinární a farmaceutické univerzity, Brno

The paper deals with chromatographic separations of newly prepared substances, aryloxyaminopropanol derivatives. The derivatives represent three homological series of four carbons and four groups of positional isomers (methyl- to butyl- in positions 2-, 3-, and 4-). Thin-layer adsorption chromatography employed the foil Silufol® UV 254 as the stationary phase and partition chromatography, commercially produced glass plates DC Fertigplatten MERCK RP-8 F254 S. High-performance liquid chromatography was used to separate positional isomers on the column SupelkosilTMABZ+PLUS. The mobile phase was methanol and acetonitrile in graded rations with water andvarious flow rates of the mobile phase were tested. Partition chromatography, determination of partition coefficient in the system octanol-water, and the values of capacity factors k' of the substances was employed to evaluate their lipophilicity.

Keywords: HPLC; TLC; positional isomers; aryloxyaminopropanol; lipophilicity

Published: March 1, 2003  Show citation