Čes. slov. farm. 2001, 50(2):83-85
Bergenia crassifolia (L.) Fritsch in vitro
- 1 Katedra farmaceutické botaniky a ekologie Farmaceutické fakulty Univerzity Karlovy
Sterile germinating plants were used to derive a tissue culture. The greatest stimulating effect on the growth of the culture was exerted by NAA cultures in all concentrations tested, by IAA inconcentrations of 1.0 and 10.0 mg.l-1, by IBA in a concentration of 0.1 mg.l-1, and by the combinationof IBA + K. The difference between the values of growth on these media was statistically insignificant. TLC analysis of callus extracts demonstrated the presence of bergenin, arbutin, hydroquinone, and methylarbutine. HPLC analysis confirmed the findings (arbutin 0.25 %, hydroquinone 0.05 %,methylarbutin 0.28 %). The results of biotransformation tests show that the highest increase inarbutin took place after addition of 4-hydroxybenzoic acid and withdrawal of the sample after 24hours (3.82 %). No transformation of arbutin or 4-methoxyphenol to produce methylarbutin tookplace in the culture under the given conditions. The highest increase in the summary content of phenolic substances occurred with the use of the elicitor Pseudomonas aeruginosa - 88 % (conc.0.0001 g/100 ml, 12-hour action).
Keywords: tissue culture; Bergenia crassifolia; biotransformation; elicitation
Published: February 1, 2001 Show citation