Čes. slov. farm. 1999, 48(5):218-220

Use of a Redox Polymers for Insulin Labelling with Technetium (^99mTc)

P. Komárek¹, I. Kleisner², M. Konopková³, I. Komárková⁴

¹ Katedra farmaceutické technologie a kontroly léčiv IPVZ, Praha

² Klinika nukleární medicíny FNKV, Praha

³ Oddělení radioizotopové diagnostiky IKEM, Praha

⁴ Oddělení radiofarmak SÚKL, Praha

The paper describes a method for porcine insulin labelling with technetium (^99mTc). When preparingthis peptide, an insoluble redox polymer on the basis of cross-linked dextran (RP G25-IDA) was usedfor labelling. The prepared kits were labelled with ^99mTc with a high labelling efficiency (more than 95 %) and stability, as assessed using methods of chromatography. ^99mTc-insulin biodistribution inrats with experimentally induced diabetes - compared with a control group - did not demonstratespecific accumulation of the labelled substance in any organ. The method for kit preparation andits labelling are mild, HPLC and SEC did not reveal any products of insulin degradation and insulincontent met the standards of Ph.B. 97. This is the first case of insulin labelling with techne-tium (⁹⁹Tc) and, also, the first use of a redox polymer for this purpose.

Keywords: insulin; redox polymer; technetium (^99mTc); radioassay; biodistribution

Published: May 1, 1999  Show citation