Čes. slov. farm. 1999, 48(3):136-139

Examination of the Activity of Some Aminopeptidases in Immobilized Cells of the Poppy Plant

J. Stano¹, K. Neubert², L. Hegedüs³, F. Andriamainty⁴

¹ Záhrada liečivých rastlín Farmaceutickej fakulty Univerzity Komenského, Bratislava

² Biochemický ústav Univerzity Martina Luthera, Halle/Saale

³ EBA, Bratislava

⁴ Katedra farmaceutickej chémie Farmaceutickej fakulty Univerzity Komenského, Bratislava

Suspension cultures of the poppy plant (Papaver somniferum L.) were permeated with Tween 80and immobilized with glutaraldehyde. The values of optimum pH of L-alanine aminopeptidase(L-Ala-AP), L-tyrosine aminopeptidase (L-Tyr-AP) and L-arginine aminopeptidase (L-Arg-AP) innative and immobilized cells are 8.0; 7.0 and 7.8, respectively. In the cells immobilized withglutaraldehyde, the activity of the enzymes under study is very low in comparison with native cells.In the immobilization of amino peptidases, alginate gel was successfully applied.

Keywords: amino peptidases; suspension cell cultures; immobilization with glutaraldehydeand alginate; Papaver somniferum

Published: March 1, 1999  Show citation