Antiadhesive, antibiofilm and dispersion activity of biosurfactants isolated from Bacillus amyloliquefaciens 3/22

Karolína Englerová, Radomíra Nemcová, Zdenka Bedlovičová, Eva Styková

Čes. slov. farm. 2021, 70(5):172-178 | DOI: 10.5817/CSF2021-5-172  

The aim of this work was to monitor the potential antibiofilm properties of biosurfactants (BS) isolated from Bacillus amyloliquefaciens 3/22 against biofilm formation of the indicator strain Staphylococcus aureus CCM 4223. In this work, the effect of BS 3/22 on biofilm growth during co-incubation, inhibition of biofilm-forming cell adhesion and biofilm dispersion was studied. BS 3/22 inhibited biofilm formation, with its formation decreasing significantly (p < 0.05; p < 0.01; p < 0.001) with increasing BS 3/22 concentration. BS 3/22 also showed antiadhesive activity, which correlated with the concentration used. The dispersing...

The effect of meloxicam and cryopreserved placenta extract on initial inflammatory response - an experimental study

Fedir V. Hladkykh

Čes. slov. farm. 2021, 70(5):179-185 | DOI: 10.5817/CSF2021-5-179  

Alteration is the first phase of the inflammatory process triggering the entire cascade of inflammation and causing destructive changes in the affected tissues. Therefore, suppression of inflammation at this point is essential for the success of anti-inflammatory therapy. Previous experimental studies have shown that the combined use of nonsteroidal anti-inflammatory drugs and drugs with pleiotropic mechanisms of action may potentiate their anti-inflammatory properties. The purpose of my work was to characterize the combined effect of cryopreserved placenta extract and meloxicam on the alterative phase of the inflammatory process in a model of aseptic...

Films from poly-γ-glutamic acid and poly-ε-lysine as the potential wound dressings - formulation, preparation and evaluation

Kamila Světlíková, Ruta Masteiková, Kateřina Tenorová, David Vetchý, Jurga Bernatonienė

Čes. slov. farm. 2021, 70(5):186-195 | DOI: 10.5817/CSF2021-5-186  

Film wound dressings represent one of the options in wound therapy. Various polymers can be used for their production. Currently, research focuses on materials of natural origin, more friendly to the human body, which are in many cases able to participate actively in the wound healing process. These include polyamino acids of bacterial origin, substances that are biodegradable, non-toxic, and have a great potential for an application not only in the medical field. From the point of view of film wound dressing formulation, poly-γ-glutamic acid (PGA), as a film-forming agent, and poly-ε-lysine (PL), characterized by antimicrobial activity,...

Use of droplet-based microfluidic techniques in the preparation of microparticles

Martina Naiserová, Jakub Vysloužil, Kateřina Kubová, Martina Holická, David Vetchý, Josef Mašek, Eliška Mašková

Čes. slov. farm. 2021, 70(5):155-163 | DOI: 10.5817/CSF2021-5-155  

Microparticles are widely used in myriad fields such as pharmaceuticals, foods, cosmetics, and other industrial fields. Compared with traditional methods for synthesizing microparticles, microfluidic techniques provide very powerful platforms for creating highly controllable emulsion droplets as templates for fabricating uniform microparticles with advanced structures and functions. Microfluidic techniques can generate emulsion droplets with precisely controlled size, shape, and composition. A more precise preparation process brings an effective tool to control the release profile of the drug and introduces an easily accessible reproducibility. The...

Telacebec (Q203): Is there a novel effective and safe anti-tuberculosis drug on the horizon?

Ivan Malík, Jozef Čižmárik, Gustáv Kováč, Mária Pecháčová, Lucia Hudecová

Čes. slov. farm. 2021, 70(5):164-171 | DOI: 10.5817/CSF2021-5-164  

High prevalence and stronger emergency of various forms of drug-resistant tuberculosis (DR-TB), including the multidrug-resistant (MDR-TB) as well as extensively drug-resistant (XDR-TB) ones, caused by variously resistant Mycobacterium tuberculosis pathogens, make first-line anti-tuberculosis (anti-TB) agents therapeutically more and more ineffective. Therefore, there is an imperative to develop novel highly efficient (synthetic) agents against both drug-sensitive-TB and DR-TB. The exploration of various heterocycles as prospective core scaffolds for the discovery, development and optimization of anti-TB drugs remains an intriguing scientific...

Laudatio RNDr. Roman Smieško - 70 rokov

Jozef Čižmárik

Čes. slov. farm. 2021, 70(5):196