Czech and Slovak Pharmacy, 2014 (vol. 63), issue 1

Editorial

Časopis Česká a slovenská farmacie vstupuje do roku 2014

Čes. slov. farm. 2014, 63(1):3

Original Articles

Production of flavonoids and isoflavonoids in jasmonic acid-induced red clover suspension cultures

Marie Kašparová, Tomáš Siatka

Čes. slov. farm. 2014, 63(1):17-21 | DOI: 10.36290/csf.2014.004

Effect of exogenously applied jasmonic acid (JA) in combination with calcium and verapamil (a calcium channels blocker) on the production of flavonoids and isoflavonoids in suspension cultures of Trifolium pratense L. was investigated. The culture was cultivated in Gamborg medium with an addition of 2 mg.l^-1 of 2,4-dichlorophenoxyacetic acid and 2 mg.l^-1 of 6-benzylaminopurine, at the temperature of 25 °C, 16-hr light/8-hr dark period. The best effect of jasmonic acid on the production of flavonoids and isoflavonoids was manifested after a 24-hour application of the 50 μmol.l^-1 concentration. The maximum production...

Study of local anaesthetics: Part 204. Determination of critical micelle concentrations of selected derivatives of pyrrolidino-m-alkoxyphenylcarbamic acid using pyrene as a probe

Jana Gališinová, Fils Andriamainty, Jozef Čižmárik, Eva Kontseková, Ivan Malík

Čes. slov. farm. 2014, 63(1):22-25 | DOI: 10.36290/csf.2014.005

The critical micelle concentrations of the studied derivatives of pyrrolidino-m-alkoxyphenylcarbamic acid in aqueous media at 25 °C were determined using the UV/VIS absorption spectroscopy method with pyrene as a probe. In the absorption spectra of the derivatives and pyrene, there were examined unmasked pyrene peaks, at wavelength of 336 and 320 nm for VIII Z, XIV Z and XVII Z, and in addition at 308 nm for XXIX Z. The critical micelle concentrations were defined from the plots of the sum of the unmasked pyrene peaks against the surfactant concentration that had a sigmoidal character of Boltzmann type. The cmc values of the derivatives were exponentially...

Inhibition of 12/15 lipoxygenase by curcumin and an extract from Curcuma longa L.

Lýdia Bezáková, Daniela Košťálová, Marek Obložinský, Peter Hoffman, Mária Pekárová, Renáta Kollárová, Ivana Holková, Silvia Mošovská, Ernest Šturdík

Čes. slov. farm. 2014, 63(1):26-31 | DOI: 10.36290/csf.2014.006

Curcumin (diferuloylmethane) is an orange-yellow secondary metabolic compound from the rhizome of turmeric (Curcuma longa L.), a spice often found in curry powder. It is one of the major curcuminoids of turmeric. For centuries, curcumin has been used in some medicinal preparations or as a food colouring agent. A variety of enzymes that are closely associated with inflammation and cancer were found to be modulated by curcumin. This paper summarized the results of the inhibitory effect of curcumin and a Curcuma longa L. ethanolic extract on lipoxygenase from the rat lung cytosolic fraction. The positional specificity determination of arachidonic...

Identifying and solving drug-related problems in terms of the community pharmacist

Lucia Masaryková, Magdaléna Fulmeková, Ľubica Lehocká, Tomáš Fazekaš

Čes. slov. farm. 2014, 63(1):32-39 | DOI: 10.36290/csf.2014.007

The issue of drug related problems (DRPs) has been known and dealt with in many studies for a long time. It is primarily due to the fact that drug-related problems have the potential to increase patients' morbidity and mortality in particular. The issue of identifying and solving drug-related problems in the area of pharmacotherapy by community pharmacists in the Slovak Republic, unlike in other European countries such as the Netherlands and the United Kingdom, is still at the beginning and gradually developing. The aim of the survey was to obtain information from pharmacy practice concerning the current state and possible solutions of potential risks...

Globalization and its impact on pharmacy services in the Slovak Republic

Ivona Malovecká, Igor Minarovič, Daniela Mináriková, Ľubica Lehocká, Miroslava Snopková, Viliam Foltán

Čes. slov. farm. 2014, 63(1):40-47 | DOI: 10.36290/csf.2014.008

Public pharmacies are excellent medical facilities having the largest number of contacts with patients. They are the facilities of the first and last contact with the health care system for the patient. Public pharmacies are unique and easily accessible places in the health care system with a high proficiency in the provision of pharmaceutical care and highly qualified medical staff. The aim of this paper was to determine geographical-demographic situation, the legal form of the ownership of public pharmacies, and the owner share of pharmacists on the capital of public pharmacies in Slovakia. The number on of providers of pharmaceutical care depends...

Review articles

Separation of amino acid enantiomers by high performance liquid chromatography

Jakub Moravčík, Katarína Hroboňová

Čes. slov. farm. 2014, 63(1):4-12 | DOI: 10.36290/csf.2014.002

Enantiomeric forms of the target analytes can be separated using chiral stationary phases based on cyclodextrins, macrocyclic polyethers and antibiotics, and derivatized polysaccharides and cyclofructans. The polar-organic, reverse-phase and normal-phase separation system of the mobile phase can be used for chromatographic separation of racemic mixtures of amino acids, depending on the type of the chiral stationary phase. In addition to the direct method of separation, the possibility of derivatization with a chiral derivatization agent is used. The conventional detection techniques, circular dichroism and laser polarimetry detection can be used to...

Where does the development of new antituberculotics go? Part 2 - In vitro evaluation

Karel Waisser

Čes. slov. farm. 2014, 63(1):13-16 | DOI: 10.36290/csf.2014.003

The present paper is a continuation of a publication of 2012 (Čes. a slov. Farm. 2012; 31, 150-158). It is oriented at the evaluation of potential antituberculotics in vitro, which is the most widely used approach to testing. The paper is based on the information from Chemical Abstracts 2011-2013. It is a selection from nearly three thousand items of information intended to help researchers. The greatest attention is paid to the multiresistant strains of Mycobacterium tuberculosis. In vitro evaluation for the development of new drugs is of great importance, but it is just the first stretch of the road to new medicinal drugs.