Czech and Slovak Pharmacy, 2006 (vol. 55), issue 4
Original Articles
Protective Effects of the Flavonoids Osajin and Pomiferin on Heart Ischemia - Reperfusion
J. Nečas, L. Bartošíková, T. Florian, J. Klusáková, V. Suchý, E. M. B. Naggar, E. Janoštíková, T. Bartošík, M. Lišková
Čes. slov. farm. 2006, 55(4):168-174 
The study was undertaken to evaluate the cardioprotective potential of the flavonoids osajin and pomiferin against ischemia-reperfusion induced injury in rat hearts as a model of antioxidant-based composite therapy. Studies were performed with isolated, modified Langendorff-perfused rat hearts and ischemia of the heart was initiated by stopping the coronary flow for 30 min followed by 60 min of reperfusion (14 ml.min-1). Wistar rats were divided into four groups. The treated groups received osajin or pomiferin (5 mg/kg/day in 0.5% Avicel), the placebo group received only 0.5 Avicel; the intact group was left without any applications. Biochemical...
Properties of Benzethonium Chloride in Micellar Solutions and the Effect of Added Sodium Chloride
F. Kopecký, B. Kopecká, P. Kaclík
Čes. slov. farm. 2006, 55(4):175-180
Aqueous solutions of the antimicrobially effective quaternary ammonium salt benzethonium chloride (hyamine 1622) were studied using UV spectrophotometry and partially conductometry. The spectra of micellar solutions of benzethonium chloride revealed a concentration-dependent bathochromic and hyperchromic shift of a weak UV absorption band in the region 250-300 nm. This served to elaborate the spectrophotometric determination of the critical micellar concentration (CMC) of benzethonium chloride and the concentration of free benzethonium cations in micellar solutions without an addition of NaCl and with a constant addition of NaCl 0.003, 0.1 and 0.15...
A Study of Qualitative Properties of the Essential Oil of Tanacetum vulgare L.
Š. Vaverková, M. Mikulášová, M. Habán, P. Sloboda
Čes. slov. farm. 2006, 55(4):181-185
The study examined the qualitative properties of the essential oil obtained from the flower heads of the species Tanacetum vulgare L. The composition of the essential oil was evaluated in plants from various localities, which differed in the altitude, temperature gradient, as well as soil-climate conditions. With the use of gas chromatography (GC/MS), 49 constituents of the essential oil were separated, out of which 35 was identified. The shares of the constituents in the essential oil differed in dependence on the locality. In the lowest-lying locality with a relatively large sunshine, the content of ß-tujons in the essential oil was the highest,...
Effect of AgNO3 on the Production of Flavonoids by the Culture of Ononis arvensis L. in vitro
L. Tůmová, D. Polívková
Čes. slov. farm. 2006, 55(4):186-188
The study tested the effect of abiotic elicitor, AgNO3, in different concentrations, on the production of flavonoids in the callus culture Ononis arvensis L. The use of this abiotic elicitor proved to be good to increase the production of flavonoids in in vitro culture. The maximal production was achieved after a 24-hour elicitation with AgNO3 in a concentration c1 (0.5 mg/l) - an increase by 934 % versus the control (without the elicitor's action).
Theory and Practice of Pharmacopoeial Control of the Quality of Drugs and Auxiliary Substances VI. Stability of Comparative Colour Solutions of the Czech Pharmacopoeia 2002
J. Šubert, O. Farsa, Z. Gajdošová
Čes. slov. farm. 2006, 55(4):189-191
Comparative colour solutions for methods I and II evaluating colouring of liquids according to PhB MMII are of identical composition. For method I, they can be stored without any time limit, whereas for method II are to be prepared just immediately prior to their use. The present paper deals with the examination of stability of comparative colour solutions PhB MMII kept in test-tubes made of colourless borosilicate glass with ground-glass stoppers, protected from light. The colour differences ΔE* in the uniform colour space CIELAB calculated from transmittance spectra measured in the course of preservation of solutions revealed that comparative...
Review articles
Naphthoquinones - biosynthesis, occurrence and metabolism in plants
P. Babula, R. Mikelová, V. Adam, R. Kizek, L. Havel, Z. Sladký
Čes. slov. farm. 2006, 55(4):151-159
Naphthoquinones are relatively widely occurring natural substances, products of secondary metabolism of some actinomycetes, fungi, lichens, and higher plants. The importance of these substances for the producers proper is, due to their wide biological activity, still discussed. In most cases they act as phytoalexines. In the case of fungi, they may play a significant role in the pathogenicity of moulds - naphthoquinones interact with mitochondria, microsomes and cytoplasmic proteins, in the form of radicals they are bound to DNA and RNA, and they do damage to them. Naphthoquinones are highly cytotoxic substances; their antimicrobial, antifungal, antiviral...
Angiotensin Converting Enzyme Inhibitors
M. Šramko, M. Remko
Čes. slov. farm. 2006, 55(4):160-167
Angiotensin converting enzyme (ACE) is a zinc metallopeptidase which plays a key role in the regulation of important vasoactive peptides through its proteolytic activity. Effective inhibitors of ACE were until recently designed in the absence of the solved 3D structure of this enzyme. About 15 ACE inhibitors are currently commercially available, all of which nonspecifically inhibit both active domains of ACE. Vasopeptidase inhibitors are mixed inhibitors of ACE and neutral endopeptidase (NEP). They contemporarily inhibit the catalytic function of two enzymes and currently are undergoing clinical trials exhibiting better efficacy in the treatment of...