Czech and Slovak Pharmacy, 2001 (vol. 50), issue 5
Articles
Advances in the Development of New Antituberculotics from the Group of OrthoCondensed Heterocyclic CompoundsPart 2. Compounds Containing More Nitrogen Atoms in the Six-Membered Ring
K. Waisser, O. Bureš, P. Holý
Čes. slov. farm. 2001, 50(5):211-218
Tuberculosis and other mycobacterial diseases are considered to be one of the most important problems of contemporary health service. Since 1985 and particularly in the 1990s and at present the search for new structures of antimycobacterial agents have ranked amon~ the foremost areas of chemotherapeutic research. The present review paper is already the 17t communication in a group of review papers about the development of new antituberculotics, the sixth about the development in retem 15 years and the second communication devoted in this series to ortho-condensed heterocyclic compounds. The classification of ortho-condensed compounds is based on six-membered...
Phytotherapeutic Aspects of the Diseases of the Circulatory SystemS. Schisandra chinensis (TURCZ.) BAILL.: Production of Drugs and Their Evaluation, Medicinal and Dietetic Preparations
L. Opletal, M. Křenková, P. Havlíčková
Čes. slov. farm. 2001, 50(5):219-224
Extracts from the fruits (seeds) of Schisandra chinensis L. and pure isolated substances are one of the components of medicinal preparations designed for the treatment of cardiovascular diseases, liver diseases, diseases of the CNS related to the old age, as a supplement in the treatment of neoplasms, diabetes, etc. They are also used for the production of nutraceuticals (soft drinks and health foods), preparations for oral hygiene and for the care for the slon and hair. The review discusses complex analytical methods used for the determination of the content of substances and the phenomena of population ecology in relation with drog production.
Biochemical and Molecular-Biological Aspects of Plant Phospholipase D
M. Obložinský, K. Weissová, L. Bezáková
Čes. slov. farm. 2001, 50(5):225-232
Phospholipase D (PLD) was hrst discovered in planu. It plays an important role in the regulation of cell functions not only in planu, but also in animal systems. It is interesting from the aspect of its dominant position in the signalling transduction processes. It controls the utilization of the membrane phospholipids for specific intentions - products of its catalytic activity are involved in intracellular communication processes. The study of the cell-regulation-system shows tkat PLD is a part of lipid-based signalling via octadecanoid pathway, which leads to the production of jasmonic acid, the basic signalling molecule in planu. PLD-mediated hydrolysis...
Studies on Local Anaesthetics CLVII. Chromatographic Properties of Pentacaine, Carbisocaine, Heptacaine, and Its 3-, and 4-Positional Isomers in the RP HPLC System
J. Čižmárik, J. Lehotay, A. Bednáriková
Čes. slov. farm. 2001, 50(5):233-237
The present study aimed to find a suitable HPLC system for the determination of the values of the capacity factor of k' pentacaine, carbisocaine, heptacaine, and its 3-, and 4-positional isomers. The column Separon SG X C-18 and a mobile phase consisting of 90 % methanol and 10 % water (with 0.5 moUl of sodium acetate) esere demonstrated to be suitable for the separation of these potential local anaesthetics from the group of phenylcarbamic acid derivatives. In this system, k'o for pentacaine is 22.9, for carbisocaine 8.6, for heptacaine 8.1, and for 3-positional derivative of heptacaine, 13.0, and 4-isomer, 18.4. It results from the data tkat log...
Cytotoxic and Genotoxic Activity of Selected Preservative Compounds for Cosmetics
S. Jantová, J. Hojerová, B. Hanusová, M. Mikulášová
Čes. slov. farm. 2001, 50(5):238-242
Cytotoxic effects of the preservative compounds for cosmetics JMAC TD, Bronopol, CA 24, and Euxyl K100 esere studied. Bronopol demonstrated the highest Cyotoxic effect on the proliferation of V79 and VH10 fibroblast cell unes - the ICioo values being 10 mg/1 during the whole experiment. The preservatives CA 24 and Euxyl K100 showed 4-times and 5-times smaller Cyotoxic activity than Bronopol ICioo = 42 or 50,3 mg/1). The preservative compounds on silver chloride ions JMAC TD manifested the lowest cytotoxicity of the preservatives tested (ICioo = 150 mg/1); 15-times smaller than Bronopol, 3,5-times smaller than CA 24 and 3-times smaller than Euxyl K100....
Unused Drugs Returned to Pharmacies II.
J. Kolář, L. Chybová, D. Holečko
Čes. slov. farm. 2001, 50(5):243-248
The problems related to unused drugs kept in households and returned to pharmacies have been investigated from various viewpoints. After an analysis of preparations which had both the primary and secondary container, or only the primary one (set I), sets II (free blisters and strips), III (free soud dosage formy which occurred singly), IV (extemporaneously prepared medicinal preparations), and V (health service appliances) were analyzed. Out of set II (1 233 blisters), 274 (22.2 %) were used and 959 (77.8 %) were still complete, unused. The expiration period was over in 66.1 % of blisters, 29.8 % was still usable, and in 4.1 % of blisters the expiration...
Effect of Chitosan on the Production of Anthracene Derivatives in the Tissue Culture of Rheum palmatum L.
M. Kašparová, T. Siatka
Čes. slov. farm. 2001, 50(5):249-253
Explant cultures have been, besides other things, used for elicitation, which is an economically advantageous method making use of protective mechanistu of plants to increase the production of secondary metabolites. The present study investigated the effect of 6, 12, 24, 48, 72, and 168 hours lasting action of four concentrations of the biotic elicitor chitosan on the production of anthracene derivatives by the callus and suspension culture ofRheum palmatum L. The culture was cultivated on a Murashige-Skoog medium with an addition of 10 mg.l-1 of a-naphthylacetic acid. A positive effect on the production was exerted in particular by elication ofthe...
Effect of Diazepam on Antidotal Therapy of Lethal Toxic Effects of Soman in the Rat
J. Kassa
Čes. slov. farm. 2001, 50(5):254-257
Experiments on male rats examined the effect of a basic antidotal therapy consisting of various types of anticholinergic substances and reactivators of acetylcholinesterase on the lethal effects of the highly toxic organophosphorous compound soman by means of determination of the medium lethal dose of soman in 24-bour survival of experimental animals. The efficacy of the basic antidotal therapy of acute intoxications by soman evaluated in this way was compared with the effect of an antidotal therapy enriched with diazepam, a drog with anticonvulsive action. The obtained results show tkat addition of diazepam to the basic antidotal therapy increases...