Čes. slov. farm. 2017, 66(2):62-66 | DOI: 10.36290/csf.2017.008
Separation of phenylalanine and methionine enantiomers by HPLC method: a comparison of stationary phase types
- 1 Ústav analytickej chémie, Fakulta chemickej a potravinárskej technológie, Slovenská technická univerzita v Bratislave, Slovenská republika
- 2 Katedra farmaceutickej chémie, Farmaceutická fakulta, Univerzita Komenského, Slovenská republika
- 3 Katedra chémie, Fakulta prírodných vied, Univerzita Sv. Cyrila a Metoda v Trnave, Slovenská republika
The paper deals with enantioselective separation of amino acids by the high performance liquid chromatography method. Separations of enantiomeric forms were tested on the chiral stationary phases based on β-cyclodextrine, isopropyl carbamate cyclofructan 6, and the macrocyclic antibiotic teicoplanin. The best enantioseparation was obtained on the teicoplanin-based chiral stationary phase in the reversed-phase mode. UV spectrophotometric detection at 210 nm was used for detection of amino acids. The method was validated with respect to linearity, precision, limit of detection, limit of quantitation, and recovery. Limits of quantitation for phenylalanine and methionine enantiomers were 0.3 and 0.2 µg.ml-1, respectively. The HPLC method with teicoplanin-based chiral stationary phase was applied for analysis of dietary supplements.
Keywords: separation of enantiomers; HPLC; phenylalanine; methionine
Grants and funding:
Práca vznikla za podpory grantu APVV-15-0355 Agentúry na podporu výskumu a vývoja Autori ďakujú prof. D.W. Armstrongovi za poskytnutie chirálnych stacionárnych fáz Chirobiotic T a IP-CF6.
Received: March 14, 2017; Accepted: April 11, 2017; Published: February 1, 2017 Show citation
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