Čes. slov. farm. 2014, 63(1):4-12 | DOI: 10.36290/csf.2014.002

Separation of amino acid enantiomers by high performance liquid chromatography

Jakub Moravčík, Katarína Hroboňová^*

Slovenská technická univerzita v Bratislave, Ústav analytickej chémie, Fakulta chemickej a potravinárskej technológie, Bratislava, Slovenská republika

Enantiomeric forms of the target analytes can be separated using chiral stationary phases based on cyclodextrins, macrocyclic polyethers and antibiotics, and derivatized polysaccharides and cyclofructans. The polar-organic, reverse-phase and normal-phase separation system of the mobile phase can be used for chromatographic separation of racemic mixtures of amino acids, depending on the type of the chiral stationary phase. In addition to the direct method of separation, the possibility of derivatization with a chiral derivatization agent is used. The conventional detection techniques, circular dichroism and laser polarimetry detection can be used to detect amino acids after HPLC separation.

Keywords: amino acids; enantiomers; chiral separations; high performance liquid chromatography

Grants and funding:

Práca vznikla za podpory grantu č. 1/0499/14 Vedeckej grantovej agentúry Ministerstva školstva Slovenskej republiky a Slovenskej akadémie vied.

Received: December 6, 2013; Accepted: December 18, 2013; Published: January 1, 2014  Show citation