JOURNAL OF THE CZECH PHARMACEUTICAL SOCIETY AND THE SLOVAK PHARMACEUTICAL SOCIETY

Čes. slov. farm. 2013, 62(6):270-275

Determination of varenicline in the preparation Champix® with the use of two-dimensional capillary electrophoresis in connection with UV detection

Juraj Piešťanský*, Katarína Maráková, Lucia Veizerová, Jaroslav Galba, Peter Mikuš
Katedra farmaceutickej analýzy a nukleárnej farmácie FaF UK, Bratislava, Slovenská republika

This paper deals with an analytical method based on two dimensional capillary electrophoresis (CITP-CZE coupling) with simple UV-detection for the determination of a highly effective drug - varenicline. The method was elaborated with the possibility of its future connection with an advanced detection ending - mass spectrometry. The electrolytes for the CITP (LE = 10 mM NH4Ac + 5 mM HAc + 0,05% m-HEC; TE = 10 mM HAc) and CZE (BGE = 20 mM HAc) separation of varenicline were selected considering such requirements. The UV detector was set at the constant wavelength of 237 nm. The presented CITP-CZE-UV combination enabled rapid and effective evaluation of varenicline in the dosage forms with LOD 5.92 ng/ml.

Keywords: nicotine; varenicline; two dimensional capillary electrophoresis; dosage forms; pharmacy

Received: October 15, 2013; Accepted: October 30, 2013; Published: June 1, 2013  Show citation

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Piešťanský J, Maráková K, Veizerová L, Galba J, Mikuš P. Determination of varenicline in the preparation Champix® with the use of two-dimensional capillary electrophoresis in connection with UV detection. Čes. slov. farm. 2013;62(6):270-275.
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