JOURNAL OF THE CZECH PHARMACEUTICAL SOCIETY AND THE SLOVAK PHARMACEUTICAL SOCIETY

Čes. slov. farm. 2009, 58(4):160-167

The role of flavonoid osajin in renal ischemia-reperfusion model

J. Nečas1,*, L. Bartošíková1, T. Bartošík2, P. Fráňa3, M. Pavlík2
1 Palacký University in Olomouc, Faculty of Medicine, Department of Physiology, Czech Republic
2 St Anne's Faculty Hospital Brno, Clinic of Anesthesiology and Resuscitation, Czech Republic
3 St Anne's Faculty Hospital Brno, IInd Clinic of Internal Medicine, Czech Republic

The aim of the present study was to investigate the antioxidant effect of the flavonoid osajin isolated from the infructences of Maclura pomifera, Moraceae, against the damage inflicted by ROS in a rat model of renal ischemia-reperfusion injury. Renal ischemia-reperfusion was induced by clamping unilateral renal artery for 60 minutes. Renal function was assessed by estimating serum creatinine, blood urea nitrogen and creatinine clearance. Renal morphological alterations were assessed by histopathological examination of hematoxylin-eosin stained sections of the kidneys. Ischemia-reperfusion produced elevated levels of malondialdehyde (a metabolic marker of lipid peroxidation), decreased the total antioxidant activity and deteriorated the renal function as assessed by increased serum creatinine, blood urea nitrogen and decreased creatinine clearance as compared to control rats. The ischemic kidneys of rats showed severe hyaline casts, epithelial swelling, proteinaceous debris, tubular necrosis, medullary hemorrhage and an extensive inflammatory infiltrate. The oral administration of osajin at the doses of 5, 10, and 20 mg/kg for 15 days significantly decreased the malondialdehyde level, increased superoxide dismutase, the total antioxidant activity and creatinine clearance to nearly the normal level. These results together with those obtained from the histopathological examination clearly demonstrate the in vivo antioxidant effect of osajin in attenuating renal ischemia-reperfusion injury.

Keywords: osajin; Maclura pomifera; antioxidant; renal ischemia-reperfusion; free oxygen radicals

Received: June 10, 2024; Accepted: July 8, 2009; Published: April 1, 2009  Show citation

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Nečas J, Bartošíková L, Bartošík T, Fráňa P, Pavlík M. The role of flavonoid osajin in renal ischemia-reperfusion model. Čes. slov. farm. 2009;58(4):160-167.
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