Čes. slov. farm. 2009, 58(2):78-82

Studies of local anesthetics Part: 187 Extraction of 1-methyl-2-piperidinoethylesters of alkoxyphenylcarbamic acids from human plasma by imprinted polymers and its comparison with classic solid phase extraction

J. Čižmárik^2,*, M. Lachová¹, J. Lehotay¹, I. Skačáni¹

¹ STU v Bratislave, Fakulta chemickej a potravinárskej technológie, Ústav analytickej chémie

² Univerzita Komenského Bratislava, Farmaceutická fakulta, Katedra farmaceutickej chémie

Molecularly imprinted polymers for 1-methyl-2-piperidinoethylester of 2-methoxyphenylcarbamic acid (template) have been synthesised. Acrylamide and methacrylic acids, respectively, were used as the functional monomers. Imprinted polymers were used as the sorbents for solid-phase extraction (MISPE). The capacity and selectivity of prepared imprinted polymers were investigated with the use of some derivatives of 1-methyl-2-piperidinethylesters of alkoxyphenylcarbamic acids. The non-imprinted (blank) polymers were prepared by the same way without a template to study the non-specific interactions. The molecularly imprinted polymer prepared from methacrylic acid was used to preconcentrate 1-methyl-2-piperidinoethylester of 2-methoxyphenylcarbamic acid and its analogues from spiked human plasma (1 μg in 1 ml of plasma).

Keywords: esters of alkoxyphenylcarbamic acids; HPLC; imprinted polymers; solid phase extraction

Grants and funding:

Táto práca vznikla vďaka finančnej podpore Grantovej agentúry Slovenskej republiky (grant 1/0058/08), APVV projekt No. 20-035-205 a VEGA projekt č.1/4291/07.

Received: March 23, 2009; Accepted: April 8, 2009; Published: February 1, 2009  Show citation