Čes. slov. farm. 2008, 57(1):17-20

Effects of auxins on growth and scopoletin accumulation in cell suspension cultures of Angelica archangelica L.

T. Siatka^*, M. Kašparová

Charles University in Prague, Faculty of Pharmacy in Hradec Králové, Czech Republic, Department of Pharmacognosy

Scopoletin is a coumarin possessing many interesting biological effects, e.g., spasmolytic, anti-inflammatory, antimutagenic, antioxidant, antifungal, apoptosis-inducing, antiproliferative, acetylcholinesterase-inhibitory, and hypouricemic activities. Plant tissue cultures represent a promising alternative source of valuable plant-derived substances. A number of physical and chemical factors influence the cell growth and secondary metabolite biosynthesis in plant tissue cultures. The mechanism of their action is not completely understood. Besides other factors, plant growth regulators and light conditions play an important role. Effects of four auxins (2,4-dichlorophenoxyacetic acid, 2,4-D, α-naphthaleneacetic acid, NAA, β-indoleacetic acid, IAA or β-indolebutyric acid, IBA) at four concentrations (0.2, 2, 10 or 20 mg/l) on the culture growth and accumulation of scopoletin in the medium were tested in Angelica archangelica cell suspension cultures cultured under continuous light or in the dark. The highest culture growth was achieved with 2 mg/l 2,4-D, and 10 mg/l IAA. The best scopoletin levels were obtained with 0.2 mg/l 2,4-D, 2 mg/l 2,4-D, 10 mg/l NAA, and 20 mg/l IAA. The effects of light conditions were less marked than those of auxins and their concentrations in influencing both the cell growth and scopoletin accumulation in Angelica archangelica cell suspension cultures. The changes brought about by auxins were modified by light conditions.

Keywords: Angelica archangelica L.; cell suspension cultures; growth; scopoletin; coumarins; auxins; light conditions; flow injection analysis

Grants and funding:

This work was supported by grant No MSM 0021620822 of the Ministry of Education, Youth and Sports of the Czech Republic.

Received: December 5, 2007; Accepted: December 17, 2007; Published: January 1, 2008  Show citation