JOURNAL OF THE CZECH PHARMACEUTICAL SOCIETY AND THE SLOVAK PHARMACEUTICAL SOCIETY

Čes. slov. farm. 2007, 56(1):21-26

Genista tinctoria in vitro

L. Tůmová, T. Šárková, J. Dušek
Univerzita Karlova v Praze, Farmaceutická fakulta v Hradci Králové, Katedra farmakognozie

The paper deals with the effects of different growth regulators on the growth of the culture and production of isoflavonoids in the cultivation of the callus culture Genista tinctoria on MS medium under varying light regimens. Of the regulators tested, the highest growth of the culture was found when using BAP 10 mg/l under continuous lighting. With the use of BAP 0.1 mg/l and under normal light regimen, the production of genistine and daidzenin was even higher than in the intact plant. Of the five isoflavonoids determined, four were demonstrated in the callus culture: genistine, genistein, daidzein, and formononetin. In the intact plant the authors managed to determine daidzein, genistein, and biochanin A.

Keywords: Genista tinctoria; callus culture; growth regulators; isoflavonoids

Received: October 25, 2006; Accepted: December 1, 2006; Published: January 1, 2007  Show citation

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Tůmová L, Šárková T, Dušek J. Genista tinctoria in vitro. Čes. slov. farm. 2007;56(1):21-26.
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