JOURNAL OF THE CZECH PHARMACEUTICAL SOCIETY AND THE SLOVAK PHARMACEUTICAL SOCIETY

Čes. slov. farm. 2005, 54(2):78-81

Arbutin, Salicin: The Possibilities of Their Biotechnological Production

J. Dušková1, J. Dušek2, L. Jahodář1, F. Poustka1
1 Univerzita Karlova v Praze, Farmaceutická fakulta v Hradci Králové, Katedra farmaceutické botaniky a ekologie
2 Univerzita Karlova v Praze, Farmaceutická fakulta v Hradci Králové, Katedra farmakognozie

The paper aimed to transform exogenous precursors with in vitro cultures of Datura meteloides, Coronilla varia, Leuzea carthamoides and Schisandra chinensis. These cultures were added the precursors of arbutin and salicin (phenylalanine, cinnamic, p-coumaric, p-anisoic, o-coumaric, salicylic acids, salicylaldehyde, helicin), not yet tested by the present authors. The culture of Schisandra chinensis was also added, besides the above-mentioned precursors, hydroquinone, because this culture had not been employed for biotransformation purposes yet. The precursors tested were used in a concentration of 100 mg.l-1 and the period of their action was 6; 12; 24; 48, and 168 hours. Positive results (both TLC and HPLC) in arbutin production were obtained in the culture of Schisandra chinensis after an addition of hydroquinone. The largest amount of arbutin in callus cultures was measured after a week's cultivation with hydroquinone (5.08 %). In this experimental variant, arbutin was released also to the culture medium. Our results revealed salicylaldehyde to be the optimal precursor of salicin. It was transformed by the culture of Datura meteloides after 6; 24, and 168 hours and by the culture of Coronilla varia after 6 hours. In comparison with arbutin, its amount was smaller.

Keywords: arbutin; salicin; precursor; biotransformation

Received: April 3, 2004; Accepted: July 1, 2004; Published: February 1, 2005  Show citation

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Dušková J, Dušek J, Jahodář L, Poustka F. Arbutin, Salicin: The Possibilities of Their Biotechnological Production. Čes. slov. farm. 2005;54(2):78-81.
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    References

    1. Mühlbach, H. P.: Biotechnol. Annu. Rev., 1998; 4, 113. Go to original source... Go to PubMed...
    2. Zenk, M. H., El-Shagi, H., Stöckigt, J. et al.: In Plant tissue culture and its biotechnological application (Bartz, W., Reinhard, E. Zenk, M. H. eds.), Berlin, Springer Verlag, 1977, s. 27. Go to original source...
    3. Dušek, J., Dušková, J., Tůmová, L., Spilková, J.: Čes. slov. Farm., 1996; 45, 209.
    4. Indrayanto, G., Zumaroh, S., Syahrani, A., Wilkins, A. L.: J. Asian Natur. Prod. Res., 2001; 3, 161. Go to original source... Go to PubMed...
    5. Syahrani, A., Panjaitan, T. S., Indrayanto, G., Wilkins, A. L.: J. Asian Natur. Prod. Res., 2000; 2, 305. Go to original source... Go to PubMed...
    6. Dušková, J., Jahodář, L., Dušek, J.: Čes. slov. Farm., 1990; 39, 452.
    7. Dušková, J., Jahodář, L., Dušek, J.: Pharmazie, 1994; 49, 624.
    8. Jahodář, L., Dušková, J., Polášek, M., Papugová, P.: Pharmazie, 1999; 54, 234.
    9. Dušková, J., Dušek, J., Jahodář, L.: Herba Pol., 1999; 45, 23.
    10. Šaršúnová, M., Schwarz, W., Michalec, Č.: Chromatografia na tenkých vrstvách vo farmácii a klinickej biochémii. 2. vyd. Martin, Osveta, 1997.
    11. Gasparič, J., Churáček, J.: Papírová a tenkovrstvá chromatografie organických sloučenin. 1. vyd. Praha, SNTL, 1981.
    12. Holopainen, M., Jahodář, L., Seppänen-Laakso, T. et al.: Acta Pharm. Fenn., 1988; 97, 197.
    13. Dušková, J., Dušek, J.: Čes. slov. Farm., 1989; 38, 257.
    14. Stöckigt, J., Obitz, P., Falkenhagen, H. et al.: Plant Cell Tissue Organ Cult., 1995; 43, 97. Go to original source...
    15. Yokoyama, M., Inomata, S., Seto, S., Yanagi, M.: Plant Cell Physiol., 1990; 31, 551.

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