Čes. slov. farm. 2004, 53(3):145-150

Oxidative Determination of Rutine in Tablets by the Method of Flow Injection Analysiswith Chemiluminescence Detection

J. Dolejšová¹, M. Polášek¹, P. Solich¹, R. Karlíček¹, L. Tůmová²

¹ Katedra analytické chemie Farmaceutické fakulty Univerzity Karlovy, Hradec Králové

² Katedra farmakognozie Farmaceutické fakulty Univerzity Karlovy, Hradec Králové

A simple, rapid and fully automated flow injection method (FIA) with chemiluminescence detectionafter oxidation with KMnO4 in acid medium at room temperature has been developed for thedetermination of rutine in pharmaceutical formulation. Hexamethaphosphate sodium was utilizedas enhancer of chemiluminescence. The calibration curvewas linear in the range 0.01-0.26 mmol.l-1with detection limit 0.005mmol.l-1,RSD 0.46% (n=10) and a sample throughput of 75.h-1 using a 200µl sample volume. The method was used for the determination of rutine in mass-produced dosageform: Rutin 250 tablets á 50 mg, (firm Nature's Bounty, USA). The FIA method was statisticallycompared with the official German Pharmacopoeia method and showed comparable accuracy, butwith the advantages of simplicity, speed and amounts of reagents consumed.

Keywords: rutine; pharmaceutical formulation; oxidation; flow injection analysis; chemiluminescence determination

Published: March 1, 2004  Show citation