Čes. slov. farm. 1998, 47(6):271-272

A New Method of Detection of Plant Extracellular Proteolytic Enzymes

J. Stano¹, I. Šafařík², P. Kovács³, K. Neubert⁴, M. Šafaříková²

¹ Záhrada liečivých rastlín Farmaceutickej fakulty Univerzity Komenského, Bratislava

² Laboratoř biochemie a biotechnologie Ústavu ekologie krajiny České akademie věd, České Budějovice

³ Katedra bunkovej a molekulárnej biológie liečiv Farmaceutickej fakulty

⁴ Biochemický ústav Univerzity Martina Luthera, Halle/Saale

By means of an insoluble chromogenic substrate (black gelasphere), a simple, sensitive and reproducible method of detection of extracellular proteolytic enzymes was worked out. Extracellular proteolytic enzymes (produced by a callus or suspension culture) hydrolyse black gelasphere. Colouring around the callus culture and under it is a sign of the activity of extracellular proteolytic enzymes produced by the callus culture. The intensity of colouring of the cultivating medium is a measure of the activity of extracellular proteolytic enzymes of the suspension culture.

Keywords: extracellular proteolytic enzymes; insoluble chromogenic substrate; black gelasphere; Chelidonium majus L.

Published: June 1, 1998  Show citation