JOURNAL OF THE CZECH PHARMACEUTICAL SOCIETY AND THE SLOVAK PHARMACEUTICAL SOCIETY

Čes. slov. farm. 2014, 63(1):17-21 | DOI: 10.36290/csf.2014.004

Production of flavonoids and isoflavonoids in jasmonic acid-induced red clover suspension cultures

Marie Kašparová*, Tomáš Siatka
Charles University in Prague, Faculty of Pharmacy in Hradec Králové, Department of Pharmacognosy, Hradec Králové

Effect of exogenously applied jasmonic acid (JA) in combination with calcium and verapamil (a calcium channels blocker) on the production of flavonoids and isoflavonoids in suspension cultures of Trifolium pratense L. was investigated. The culture was cultivated in Gamborg medium with an addition of 2 mg.l-1 of 2,4-dichlorophenoxyacetic acid and 2 mg.l-1 of 6-benzylaminopurine, at the temperature of 25 °C, 16-hr light/8-hr dark period. The best effect of jasmonic acid on the production of flavonoids and isoflavonoids was manifested after a 24-hour application of the 50 μmol.l-1 concentration. The maximum production of JA-induced suspension culture was observed when cells were treated with a high level of calcium (10 mmol.l-1). The addition of all concentrations of verapamil to JA-induced suspension culture decreased production of flavonoids and isoflavonoids.

Keywords: flavonoids; isoflavonoids; jasmonic acid; elicitation; Trifolium pratense suspension culture
Grants and funding:

This study was financially supported by the grant of Charles University in Prague SVV 267 004.

Received: November 6, 2013; Accepted: December 19, 2013; Published: January 1, 2014  Show citation

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Kašparová M, Siatka T. Production of flavonoids and isoflavonoids in jasmonic acid-induced red clover suspension cultures. Čes. slov. farm. 2014;63(1):17-21. doi: 10.36290/csf.2014.004.
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