JOURNAL OF THE CZECH PHARMACEUTICAL SOCIETY AND THE SLOVAK PHARMACEUTICAL SOCIETY

Čes. slov. farm. 2017, 66(1):15-22 | DOI: 10.36290/csf.2017.003

Cytotoxic, anti-cancer, and anti-microbial effects of different extracts obtained from Artemisia rupestris

Shamshabanu Nokerbek1, Zuriyadda Sakipova1, Marta Chalupová2, Marcela Nejezchlebová3, Jan Hošek3,*
1 Department of Pharmaceutical Technology, Faculty of Pharmacy, Kazakh National Medical University, Kazakhstan
2 Department of Human Pharmacology and Toxicology, Faculty of Pharmacy, University of Veterinary and Pharmaceutical Sciences Brno, Czech Republic
3 Department of Molecular Biology and Pharmaceutical Biotechnology, Faculty of Pharmacy, University of Veterinary and Pharmaceutical Sciences, Brno, Czech Republic

Artemisia rupestris is a part of traditional Kazakh folk medicine. Extracts obtained from this plant are used to treat various diseases, including cancer. This study evaluates the anti-microbial, cytotoxic, and anti-cancer effects of different extracts of the plant. Different extraction techniques were used and the resultant activities were compared. Extracts of A. rupestris were prepared from the flowers plus the leaves and from the stems. The antimicrobial activity against Candida albicans and Staphylococcus aureus was quantified. Cell lines L1210 and THP-1 were used to evaluate the cytotoxic potential of these extracts in vitro. The anti-cancer effect was tested using L1210-induced tumorgenesis in mouse model. The aqueous extract of stems was the most active against C. albicans, whereas the methanolic extract of flowers plus leaves especially inhibited the growth of S. aureus. The aqueous extracts were found to be non-cytotoxic for both cell lines, whereas the lipophilic extracts showed cytotoxic effects. The extract obtained from flowers plus leaves was more cytotoxic than that from stems. The tested extracts showed no anti-cancer potential. The results obtained testify to the relatively safe consumption of aqueous extracts of A. rupestris, but lipophilic extracts showed toxic effects and their consumption should be considered more carefully.

Keywords: L1210 cell line; THP-1 cell line; microwave-assisted extraction; ultrasonic-assisted extraction; Candida albicans; Staphylococcus aureus

Received: January 12, 2017; Accepted: March 10, 2017; Published: January 1, 2017  Show citation

ACS AIP APA ASA Harvard Chicago Chicago Notes IEEE ISO690 MLA NLM Turabian Vancouver
Nokerbek S, Sakipova Z, Chalupová M, Nejezchlebová M, Hošek J. Cytotoxic, anti-cancer, and anti-microbial effects of different extracts obtained from Artemisia rupestris. Čes. slov. farm. 2017;66(1):15-22. doi: 10.36290/csf.2017.003.
Share...
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)
    • Open full article

    References

    1. Wei X., Aibai S. Progresses in the research of Artemisia rupestris L. Chin. Nat. Pharm. J. 2008; 6, 61-66.
    2. Yu D. Q. Recent results on structural chemistry of new natural products from Chinese herbal medicine. Pure and Appl. Chem. 1998; 70, 431-434. Go to original source...
    3. Wei X., Aibai S., Li Z. J. Study on the anti-inflammatory effect of the extract of Artemisia rupestris L. Lishizhen Med. Mat. Med. Res. 2008; 19, 2836-2838.
    4. Wang R. Kazakh folk medicine. Urumqi, China: Xinjiang Science-technical Publishing House 2008.
    5. Hou J., Dong H., Yan M., Zhu F., Zhang X., Wang H., Ye W., Li P. New guaiane sesquiterpenes from Artemisia rupestris and their inhibitory effects on nitric oxide production. Bioorg. Med. Chem. Lett. 2014; 24, 4435-4438. Go to original source... Go to PubMed...
    6. Liu X. C., Li Y. P., Li H. Q., Deng Z. W., Zhou L., Liu Z. L., Du S. S. Identification of repellent and insecticidal constituents of the essential oil of Artemisia rupestris L. aerial parts against Liposcelis bostrychophila Badonnel. Molecules 2013; 18, 10733-10746. Go to original source... Go to PubMed...
    7. Yong J., Lu C., Aisa H. A. Advances in studies on the rupestonic acid derivatives as anti-influenza agents. Mini Rev. Med. Chem. 2013; 13, 310-315. Go to original source...
    8. Batay Z. Xinjiang medicinal plants. Urumqi, China: Xinjiang Science-technical Publishing House 2006.
    9. Carlo B., Carlotta F., Tommaso S. Essential oils of three Asiatic Artemisia species. Photochemistry 1985; 24, 2440-2442. Go to original source...
    10. Su Z., Wu H., Yang Y., Aisa H. A., Slukhan U., Aripova S. Preparative isolation of guaipyridine sesquiterpene alkaloid from Artemisia rupestris L. flowers using high-speed counter-current chromatography. J. Sep. Sci. 2008; 31, 2161-2166. Go to original source... Go to PubMed...
    11. Zhen S., Han K. W., He F., Slukhan U., Aisa H. A. New Guaipyridine Sesquiterpene Alkaloids from Artemisia rupestris L.. Helv. Chim. Acta 2010; 93, 33-38. Go to original source...
    12. Gu D., Yang Y., Abdulla R., Aisa H. A. Characterization and identification of chemical compositions in the extract of Artemisia rupestris L. by liquid chromatography coupled to quadrupole time-of-flight tandem mass spectrometry. Rapid Commun. Mass Spectrom. 2012; 26, 83-100. Go to original source... Go to PubMed...
    13. He F., Aisa H. A., Shakhidoyatov K. M. Flavones from Artemisia rupestris. Chem. Nat. Compd. 2012; 48, 685-686. Go to original source...
    14. Xie Z. Y., Lin T. T., Yao M. C., Wan J. Z., Yin S. Unusual Guaiane Sesquiterpenoids from Artemisia rupestris. Helv. Chim. Acta 2013; 96, 1182-1187. Go to original source...
    15. Razzaghi-Abyaneh M., Rai M. Antifungal Metabolites from Plants. Berlin, Germany: Springer-Verlag Berlin Heidelberg 2013. Go to original source...
    16. Poiată A., Tuchiluş C., Ivănescu B., Lazăr M. I. Antibacterial activity of some Artemisia species extract. Rev. Med. Chir. Soc. Med. Nat. Iasi 2009; 113, 911-914.
    17. Karabegović I., Nikolova M., Veličković D., Stojičević S., Veljković V., Lazić M. Comparison of Antioxidant and Antimicrobial Activities of Methanolic Extracts of the Artemisia sp. Recovered by Different Extraction Techniques. Chin. J. Chem. Eng. 2011; 19, 504-511. Go to original source...
    18. Hall I. H., Tolmie C. E., Barnes B. J., Curtis M. A., Russell J. M., Finn M. G., Grimes R. N. Cytotoxicity of tantalum(V) and niobium(V) small carborane complexes and mode of action in P388 lymphocytic leukemia cells". Appl. Organomet. Chem. 2000; 14, 108-118. Go to original source...
    19. Evans M. A., Smith D. C., Holub J. M., Argenti A., Hoff M., Dalglish G. A., Wilson D. L., Taylor B. M., Berkowitz J. D., Burnham B. S., Krumpe K., Gupton J. T., Scarlett T. C., Durham Jr. R. W., Hall I. H. Synthesis and cytotoxicity of substituted ethyl 2-phenacyl-3-phenylpyrrole-4-carboxylates. Arch Pharm (Weinheim) 2003; 336, 181-190. Go to original source... Go to PubMed...

    Return to the content


    Czech and Slovak Pharmacy

    Madam, Sir,
    please be aware that the website on which you intend to enter, not the general public because it contains technical information about medicines, including advertisements relating to medicinal products. This information and communication professionals are solely under §2 of the Act n.40/1995 Coll. Is active persons authorized to prescribe or supply (hereinafter expert).
    Take note that if you are not an expert, you run the risk of danger to their health or the health of other persons, if you the obtained information improperly understood or interpreted, and especially advertising which may be part of this site, or whether you used it for self-diagnosis or medical treatment, whether in relation to each other in person or in relation to others.

    I declare:

    1. that I have met the above instruction
    2. I'm an expert within the meaning of the Act n.40/1995 Coll. the regulation of advertising, as amended, and I am aware of the risks that would be a person other than the expert input to these sites exhibited

    No
    Yes

    If your statement is not true, please be aware
    that brings the risk of danger to their health or the health of others.