JOURNAL OF THE CZECH PHARMACEUTICAL SOCIETY AND THE SLOVAK PHARMACEUTICAL SOCIETY

Čes. slov. farm. 2013, 62(1):40-45

Testing of the potentially probiotic lactobacilli for use in food supplements

Andrea Bilková*, František Bilka, Hana Kiňová Sepová, Andrea Balažová
Univerzita Komenského v Bratislave, Farmaceutická fakulta, Katedra bunkovej a molekulárnej biológie liečiv, Bratislava, Slovenská republika

Some lactobacilli strains are used as probiotics in food industry and in dietary supplements. Eight lactobacilli strains, originated from the stomach of the lamb and goatling, are tested for their potential use in human and/or veterinary medicine in our department. Sanguinarine is a major alkaloid of Papaveraceae suspension cultures. For its antimicrobial, antifungal, anti-plaque and anti-inflammatory properties sanguinarine found application in dental preparations. A mixture of sanguinarine with other alkaloids is used for appetite stimulation of livestock. This paper is focused on testing of selected lactobacilli sensitivity to sanguinarine, isolated from the opium poppy suspension cultures. L. reuteri KO5 demonstrated the lowest sensitivity (MIC 0.6 mg.l-1). Other less sensitive strains were L. murinus C, L. mucosae D and L. plantarum KG4 (MIC 0.3 mg.l-1). For a combination of more strains of microorganisms in one preparation it is necessary to know their ability to influence their growth and survival. To test the mutual influence of lactobacilli, the streak line method on agar plates was used. The results show that the strains L. reuteri E, L. plantarum KG1 a L. reuteri KO4m are the most suitable ones for the use in combinations.

Keywords: Lactobacillus spp.; probiotics; food supplements; sanguinarine

Received: November 23, 2012; Accepted: January 14, 2013; Published: January 1, 2013  Show citation

ACS AIP APA ASA Harvard Chicago Chicago Notes IEEE ISO690 MLA NLM Turabian Vancouver
Bilková A, Bilka F, Kiňová Sepová H, Balažová A. Testing of the potentially probiotic lactobacilli for use in food supplements. Čes. slov. farm. 2013;62(1):40-45.
Share...
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)
    • Open full article

    References

    1. Coudeyras S., Jugie G., Vermerie M., Forestier Ch. Adhesion of human probiotic Lactobacillus rhamnosus to cervical and vaginal cells and interaction with vaginosis-associated pathogens. Infect. Dis. Obstet. Gynecol. 2008; http://www.hindawi.com/journals/idog/2008/549640/(13.11.2012) Go to original source...
    2. Myllyluoma E., Ahonen A. M., Korpela R., Vapaatalo H., Kankuri E. Effects of multispecies probiotic combination on Helicobacter pylori infection in vitro. Clin. Vaccine Immunol. 2008; 15, 1472-1482. Go to original source... Go to PubMed...
    3. Lin P. P., Hsieh Y. M., Tsai Ch. Ch. Antagonistic activity of Lactobacillus acidophilus RY2 isolated from healthy infancy feces on the growth and adhesion characteristics of enteroaggregative Escherichia coli. Anaerobe 2009; 15, 122-126. Go to original source... Go to PubMed...
    4. Sepp E., Stšepetova J., Smidt I., Rätsep M., Kõljalg S., Lõivukene K., Mändar R., Jaanimäe L., Löhr H.I., NatČs O.B., Naaber P. Intestinal lactoflora in Estonian and Norwegian patients with antibiotic associated diarrhea. Anaerobe 2011; 17, 407-409. Go to original source... Go to PubMed...
    5. Bilková A., Kiňová Sepová H., Bilka F., Balažová A. Bakteriocíny produkované baktériami mliečneho kvasenia. Čes. a slov. Farm. 2011; 60, 65-72.
    6. Kiňová Sepová H., Dubničková M., Bilková A., Bukovský M., Bezáková L. Identification and biological activity of potential probiotic bacterium isolated from the stomach mucus of breast-fed lamb. Braz. J. Microbiol. 2011; 42, 1188-1196. Go to original source...
    7. Nikawa H., Makihira S., Fukushima H., Nishimura H., Ozaki Y., Ishida K., Darmawan S., Hamada T., Hara K., Matsumoto A., Takemoto T., Aimi R. Lactobacillus reuteri in bovine milk fermented decreases the oral carriage of mutans streptococci. Int. J. Food Microbiol. 2004; 95, 219-223. Go to original source... Go to PubMed...
    8. Caglar E., Kavaloglu S. C., Kuscu O. O., Sandalli N., Holgerson P. L., Twetman S. Effect of chewing gums containing xylitol or probiotic bacteria on salivary mutans streptococci and lactobacilli. Clin. Oral Investig. 2007; 11, 425-429. Go to original source... Go to PubMed...
    9. Ouwehand A. C., Vesterlund S. Antimicrobial components from lactic acid bacteria. In: Salminen S., von Wright A., Ouwehand A. C. eds. Lactic Acid Bacteria: Microbiological and functional aspects, 3rd ed. New York: Marcel Dekker, Inc. 2004. Go to original source...
    10. Kiňová Sepová H., Bilková A., Bilka F., Bezáková L. Antimikróbne pôsobiace látky produkované baktériami mliečneho kvasenia. Čes. a slov. Farm. 2010; 59, 155-159.
    11. Bilková A., Bilka F., Bezáková L. Enzymológia tvorby benzylizochinolínových alkaloidov. Čes. slov. Farm. 2005; 54, 17-22.
    12. Dostál J., Slavík J. Novější poznatky o sanguinarinu a příbuzných alkaloidech. Chem. Listy 2000; 94, 15-20.
    13. Zdařilová A., Malíková J., Dvořák Z., Ulrichová J., Šimánek V. Kvartérní isochinolinové alkaloidy sanguinarin a chelerythrin. Účinky in vitro a in vivo. Chem. Listy 2006; 100, 30-41.
    14. Simeon S., Rios J. L., Villar A. Pharmacological activities of benzophenanthridine and phenanthrene alkaloids. Pharmazie 1989; 44, 593-597.
    15. Walterová D., Ulrichová J., Valka I., Vičar J., Vavrečková C., Táborská E., Harkrader R. J., Meyer D. L., Černá H., Šimánek V. Benzo[c]phenanthridine alkaloids sanguinarine and chelerythrine: Biological activities and dental care applications. Acta Univ. Palacky Olomouc Fac. Med. 1995; 139, 7-16.
    16. Newton S. M., Lau C., Gurcha S. S., Besra G. S., Wright C. W. The evaluation of fourty-three plant species for in vitro antimicrobial activities. J. Ethnopharmacol. 2002; 79, 57-67. Go to original source... Go to PubMed...
    17. Mahady G. B., Pendland S. L., Stoia A., Chadwick L. R. In vitro susceptibility of Helicobacter pylori to isoquinoline alkaloids from Sanguinaria canadensis and Hydrastis canadensis. Phytother. Res. 2003; 17, 217-221. Go to original source... Go to PubMed...
    18. Merschjohann K., Sporer F., Steverding D., Wink M. In vitro effect of alkaloids on bloodstream forms of Trypanosoma brucei and T. congolense. Planta Med.2001; 67, 623-627. Go to original source... Go to PubMed...
    19. Navarro V., Delgado G. Two antimicrobial alkaloids from Bocconia arborea. J. Ethnopharmacol. 1999; 66, 223-226. Go to original source... Go to PubMed...
    20. Bilková A., Kiňová Sepová H., Bukovský M., Bezáková L. Antibacterial potential of lactobacilli isolated from a lamb. Vet. Med. 2011; 56, 319-324. Go to original source...
    21. Balažová A., Blanáriková V., Bilka F., Bilková A., Kiňová Sepová, H. Efekt troch rôznych elicitorov na produkciu sanguinarínu suspenznými kultúrami nízko-morfínovej odrody maku siateho (Papaver somniferum L.). Čes. slov. Farm. 2011; 60, 237-240.
    22. Bilková A., Kiňová Sepová H., Bilka F., Bukovský M., Balažová A., Bezáková, L. Identification of newly isolated lactobacilli from the stomach mucus of lamb. Acta Facult. Pharm. Univ. Comenianae 2008; 55, 64-72.
    23. Kiňová Sepová H., Bilková A. Isolation and identification of new lactobacilli from goatling stomach and investigation of reuterin production in Lactobacillus reuteri strains. Folia Microbiol 2003; 58, 33-38. Go to original source... Go to PubMed...
    24. Mlynarčík D., Bukovský M., Čupková V., Sirotková L. Praktické cvičenia z mikrobiológia a imunologických prípravkov. Skriptá, FaF UK Bratislava, 1995, 160.
    25. Annuk H., Shchepetova J., Kullisaar, T., Songisepp E., Zilmer M., Mikelsaar M. Characterization of intestinal lactobacilli as putative probiotic candidates. J. Appl. Microbiol. 2003; 94, 403-412. Go to original source... Go to PubMed...
    26. Hütt P., Shchepetova J., Lõivukene K., Kullisaar T., Mikelsaar M. Antagonistic activity of probiotic lactobacilli and bifidobacteria against entero- and uropathogens. J. Appl. Microbiol. 2006; 100, 1324-1332. Go to original source... Go to PubMed...
    27. Vieira S. L., Oyarzabal O. A., Freitas D. M., Berres J., Pena J. E. M., Torres C. A. Performance of broilers fed diets supplemented with sanguinarine-like alkaloids and organic acid. J. Appl. Poult. Res. 2008; 17, 128-133. Go to original source...
    28. Rawling M. D., Merrifield D. L., Davies S. J. Preliminary assessment of dietary supplementation of Sangrovit on red tilapia (Oreochromis niloticus) growth performance and health. Aquaculture 2009; 294, 118-122. Go to original source...
    29. Jurkiewicz J., Gruzauskas R., Zdunczyk Z., Semaskaite A., Jankowski J., Totilas Z., Jarule V., Sasyte V., Zdunczyk P., Raceviciute-Stupeliene A., Svirmickas G. Effect of dietary addition of Macleaya cordata alkaloid extract on growth performance, caecal indices and breast meat fatty acids profile in male broilers. J. Anim. Physiol. Anim. Nutr. 2011; 95, 171-178. Go to original source... Go to PubMed...
    30. Zdařilová A., Vrublová E., Vostalová J., Klejdus B., Stejskal D., Prosková J., Kosina P., Svobodová, A., Večera R., Hrbáč J., Černochová D., Vičar J., Ulrichová J., Šimánek V. Natural feed additive of Macleaya cordata: safety assessment in rats a 90-day feeding experiment. Food Chem Toxicol. 2008; 46, 3721-3726. Go to original source... Go to PubMed...
    31. Nousiainen J., Javanainen P., Setala J., von Wright A. Lactic acid bacteria as animal probiotics. In: Salminen S., Ouwehand A., von Wright A. eds. Lactic acid bacteria: microbial and functional aspects 3rd ed. New York: Marcel Dekker 2004. Go to original source...
    32. Caglar E., Sandalli N., Twetman S., Kavaloglu S., Ergeneli S., Selvi S. Effect of yogurt with Bifidobacterium DN-173 010 on salivary mutans streptococci and lactobacilli in young adults. Acta Odontol. Scand. 2005; 63, 317-320. Go to original source... Go to PubMed...
    33. Caglar E., Cildir S. K., Ergeneli S., Sandalli N., Twetman S. Salivary mutans streptococci and lactobacilli levels after ingestion of the probiotic bacterium Lactobacillus reuteri ATCC 55730 by straws or tablets. Acta Odontol. Scand. 2006; 64, 314-318. Go to original source... Go to PubMed...
    34. Tong H., Chen W., Merritt J., Qi F., Shi W., Dong X. Streptococcus oligofermentans inhibits Streptococcus mutans through conversion of lactic acid into inhibitory H2O2: a possible counteroffensive strategy for interspecies competition. Mol. Microbiol. 2007; 63, 872-880. Go to original source... Go to PubMed...
    35. He X., Lux R., Kuramitsu H. K., Anderson M. H., Shi W. Achieving probiotic effects via modulating oral microbial ecology. Adv. Dent. Res. 2009; 21, 53-56. Go to original source... Go to PubMed...
    36. Tanzer J., Livingston J., Thompson A. The microbiology of primary dental caries in human. J. Dent. Educ. 2001; 65, 1028-1037. Go to original source...
    37. Švec P., Sedláček I., Žáčková L., Nováková D., Kukletová M. Lactobacillus spp. associated with early childhood caries. Folia Microbiol. 2009; 54, 53-58. Go to original source... Go to PubMed...

    Return to the content


    Czech and Slovak Pharmacy

    Madam, Sir,
    please be aware that the website on which you intend to enter, not the general public because it contains technical information about medicines, including advertisements relating to medicinal products. This information and communication professionals are solely under §2 of the Act n.40/1995 Coll. Is active persons authorized to prescribe or supply (hereinafter expert).
    Take note that if you are not an expert, you run the risk of danger to their health or the health of other persons, if you the obtained information improperly understood or interpreted, and especially advertising which may be part of this site, or whether you used it for self-diagnosis or medical treatment, whether in relation to each other in person or in relation to others.

    I declare:

    1. that I have met the above instruction
    2. I'm an expert within the meaning of the Act n.40/1995 Coll. the regulation of advertising, as amended, and I am aware of the risks that would be a person other than the expert input to these sites exhibited

    No
    Yes

    If your statement is not true, please be aware
    that brings the risk of danger to their health or the health of others.