Čes. slov. farm. 2011, 60(1):7-10

Study on ^99mTc-MAG3 and ^99mTc-DMSA renal accumulation using in vitro cellular model

Zbyněk Nový, Jana Mandíková, František Trejtnar^*

Charles University in Prague, Faculty of Pharmacy, Department of Pharmacology and Toxicology, Hradec Králové, Czech Republic

Mercaptoacetyltriglycine (MAG3) and dimercaptosuccinic acid (DMSA) labelled with technetium-99m belongs to standard renal radiodiagnostics. However, the renal transport mechanisms responsible for their high renal uptake have not been fully explained. In addition, no in vitro experimental study comparing the renal uptake of these radiopharmaceuticals at the cellular level has not been performed. The investigation compared the ^99mTc-MAG3 and ^99mTc-DMSA renal uptake using primary rat renal cells and evaluated contribution of active and passive transport processes to the renal accumulation. The renal cells were isolated from the rat kidneys by means of the two-phase collagenase perfusion method. The used experimental model showed to be useful tool for such type of investigation. The results documented significant quantitative and qualitative differences in the accumulation of ^99mTc-DMSA and ^99mTc-MAG3 in the rat isolated cells. The found experimental data indicated several times higher uptake of ^99mTc-MAG3 than that found in ^99mTc-DMSA. ^99mTc-MAG3 cellular uptake was substantially decreased when active, energy-dependent processes were inhibited. However, ^99mTc-DMSA accumulation in the renal cells demonstrated only a minor dependency on energy. These findings demonstrate a very different character of the membrane transport determining ^99mTc-DMSA and ^99mTc-MAG3 renal accumulation.

Keywords: renal radiodiagnostics; membrane transport; bioelimination

Grants and funding:

This work was supported by grant No. 124409/FaF/C-LEK of the Grant Agency of Charles University

Received: November 30, 2010; Accepted: December 13, 2010; Published: January 1, 2011  Show citation