JOURNAL OF THE CZECH PHARMACEUTICAL SOCIETY AND THE SLOVAK PHARMACEUTICAL SOCIETY

Čes. slov. farm. 2002, 51(2):91-95

Validation of a HPLC Method for the Determination of Dioxopromethazine and Phenylephrine in Eye Drops

T. Hudecová, ©. Hatrík, N. Zimová, E. Havránek
Katedra farmaceutickej analýzy a nukleárnej farmácie Farmaceutickej fakulty Univerzity Komenského, Bratislava

The present paper introduces a rapid HPLC method for the determination of dioxopromethazine and phenylephrine in eye drops. The method uses a modified C18 stationary phase optimized for the separation of basic compounds and a methanoUl.5 mM phosphoric acid (60/40 v/v, pH 3.02) mobile phase. The flow rate is set to 2 mUmin, sample volume 20 pl, and compounds are detected at 275 nm. Prior to analysis, the eye drops are diluted with water in a rado of 1:50. The elaborated HPLC method and the chromatographic system esere validated according to the procedure for the validation of chromatographic systems and methds.

Keywords: HPLC, UC; dioxopromethazine; phenylephrine; eye drops; validation

Published: February 1, 2002  Show citation

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Hudecová T, Hatrík ©, Zimová N, Havránek E. Validation of a HPLC Method for the Determination of Dioxopromethazine and Phenylephrine in Eye Drops. Čes. slov. farm. 2002;51(2):91-95.
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