Čes. slov. farm. 2003, 52(2):97-101

Effect of the Saccharide Parts in the Teikoplanin Stationary Phase on the Separation ofSome Enantiomers of the Phenylcarbamic Type by the HPLC Method

T. Rojkovičová¹, J. Lehotay¹, J. Čižmárik²

¹ Katedra analytickej chémie Fakulty chemickej a potravinárskej technológie Slovenskej technickej univerzity, Bratislava

² Katedra farmaceutickej chémie Farmaceutickej fakulty Univerzity Komenského, Bratislava

The paper presents the results obtained with the use of two chiral stationary phases (CSP) basedon a macromolecular antibiotic agent - teikoplanin containing saccharide parts (CHIROBIOTIC T)and teikoplanin without saccharide parts (CHIROBIOTIC TAG). Racemic mixtures of 1-methyl-2--piperidinoethyl esters of 2-, 3-, and 4-alkoxyphenylcarbamic acids were examined. The investigationincluded interactions between separated substances and CSP, and the effect of separation of theenantiomers under study on the value of the differentiation factor (Rij) under identical chromatographicconditions with the use of the method of high-performance liquid chromatography (HPLC).On the basis of the obtained results, it is possible to report that CSP-CHIROBIOTIC TAG is moreadvantageous for the substances of the type of 1-methyl-2-piperidinoethyl esters of 2-, 3-, and 4-alkoxyphenylcarbamic acids, because it does not contain a saccharide part, which decreases the possibility of non-polar interactions which exert a negative effect on the Rij value.

Keywords: teikoplanin; TAG; plenylcarbamic acids; HPLC; separation; separation ofenantiomers; local anaesthetics

Published: February 1, 2003  Show citation